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Protein-polymer-hydrogels

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Protein polymer hydrogels

Details

Inventors: Battista, Orlando A.;
Assignee:
Primary Examiner: Schain; Howard E.
Assistant Examiner:
Attorney, Agent or Firm: Mueller; George F.

Protein hydrogel structures formed from natural proteins having an average molecular weight of about 100,000 or less by dissolving the protein in an aqueous acidic solution, subjecting the protein to vapor phase crosslinking with at least two crosslinking agents, and air drying to a moisture content not exceeding 10 percent. The drying may be effected by treatment with a water-miscible organic solvent or by air drying. The structures may be in the form of soft contact lenses, films, fibers, and prosthetics.

DETAILED DESCRIPTION What is claimed is: 1.
The method of preparing a three-dimensional structure comprising preparing an aqueous acidic solution of a non-crystalline, natural animal or vegetable protein polymer or mixtures thereof, the polymers having an average molecular weight not exceeding 100,000, shaping the solution into a three-dimensional structure, subjecting the structure to a vapor phase crosslinking reaction using at least two crosslinking agents in vaporized state to form a crosslinked polymer structure, subjecting the structure to washing with water, subjecting the washed structure to dehydration by immersion in water-miscible organic solvent mixture, subjecting the structure to washing with water and drying the structure to a moisture content of not more than 10 percent.
2.
The method as defined in claim 1 wherein the solution of the protein contains at least one crosslinking agent.
3.
The method as defined in claim 1 wherein following the vapor phase crosslinking reaction the structure is immersed in a water-organic solvent mixture containing at least 1 crosslinking agent.
4.
The method as defined in claim 1 wherein during the vapor phase crosslinking reaction the structure is subjected to UV and/or gamma radiation.
5.
The method as defined in claim 1 wherein following the vapor phase crosslinking reaction the structure is immersed in a water-organic solvent mixture containing at least 1 crosslinking agent and during both crosslinking reactions the structure is subjected to UV and/or gamma radiation.
6.
The method as defined in claim 1 wherein the aqueous acidic solution of the protein polymer has a pH of from about 3.
5 to about 5.
5.
7.
The method as defined in claim 1 wherein the protein polymer is an animal protein.
8.
The method as defined in claim 1 wherein the protein polymer is gelatin.
9.
The method as defined in claim 1 wherein the protein polymer is a vegetable protein.
10.
The method as defined in claim 1 wherein the aqueous acidic solution of the protein polymer is an aqueous hydrochloric acid solution of animal protein and has a pH of from about 3

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