Home | Links | Contact Us | More About Intellectual Property | Bookmark
Search patents:
Home Drugs DNA-comprising-regulatory-regions-from-gene-y-of-penicillium-chrysogenum

 DNA comprising regulatory regions from gene y of penicillium chrysogenum

Details
Inventors: Bovenberg, Roelof Ary Lans; Vollebregt, Adrianus Wilhelmus Hermanus; Van Solingen, Pieter;
Assignee: Gist-Brocades, NV. (NL)
Primary Examiner: Ulm; John
Assistant Examiner:
Attorney, Agent or Firm: McDonnell Boehnen Hulbert & Berghoff

A gene expression system and a protein secretion system for eukaryotic, especially fungal, microorganisms has been provided for.

DETAILED DESCRIPTION What is claimed is: 1.
An isolated DNA comprising a nucleotide sequence obtainable from gene Y which regulates transcription initiation of a DNA sequence of interest operably joined to said nucleotide sequence.
2.
The isolated DNA according to claim 1, wherein said nucleotide sequence comprises nucleotide 1 up to nucleotide 426 of SEQ ID NO: 1.
3.
An isolated DNA comprising a nucleotide sequence obtainable from gene Y which regulates transcription termination of a DNA sequence of interest operably joined to said nucleotide sequence.
4.
The isolated DNA according to claim 3, wherein said nucleotide sequence comprises nucleotide 850 up to nucleotide 1514 of SEQ ID NO: 1.
5.
An isolated DNA comprising a nucleotide sequence obtainable from gene Y which encodes a secretion signal sequence.
6.
The isolated DNA according to claim 5, wherein said nucleotide sequence comprises nucleotide 427 up to nucleotide 537 of SEQ ID NO: 1.
7.
A DNA construct comprising a first nucleotide sequence obtainable from gene Y which regulates transcription initiation, a second nucleotide sequence obtainable from gene Y which regulates transcription termination, and a DNA sequence of interest operably fused to said first and second nucleotide sequences wherein at least one of said first and second nucleotide sequences and said DNA sequence of interest does not occur naturally with one or more of the remaining sequences.
8.
The DNA construct according to claim 7, wherein said DNA construct further comprises a third nucleotide sequence obtainable from gene Y and encoding a secretion signal sequence operably joined between said first nucleotide sequence and said DNA sequence of interest.
9.
A method for obtaining expression and secretion of a protein encoded by a DNA sequence of interest, said method comprising: growing a host containing a DNA construct according to claim 8, under conditions whereby said protein is expressed and secreted.
10.
A method for obtaining expression of a DNA sequence of interest, said method comprising: growing a host containing a DNA construct according to claim 7, under conditions whereby said DNA sequence of interest is expressed



Related patents
  High molecular weight collagen-like protein polymers
What is claimed is: 1. An unnatural collagen-like polymer of at least about 30 kD, characterized by: being capable of being expressed in a unicellular organism from a ...

0.014

Archive: All patents - Links

Copyright (c)2006 Eipa-patents.org - All rights reserved