Isolated nucleic acid encoding seven transmembrane receptors

DETAILED DESCRIPTION The present invention is illustrated by the following examples relating to the isolation of human genomic and cDNA sequences encoding the novel 7TM receptors herein designated V28, V31, V112, R20, R2, R12 and RM3.
More particularly, Example 1 describes the isolation of PCR fragments encoding portions of the R20, V31, V28 and V112 7TM receptors.
Example 2 describes the isolation of a full length human V31 genomic clone.
Example 3 describes the isolation of a V31 human cDNA clone and further characterization of the V31 genomic clone.
Example 4 presents experiments which reveal the chromosomal location of the human V31 gene.
Example 5 describes the cloning of a full length murine V31 genomic clone.
The cloning of a full length, human genomic clones for V28 is described in Example 6.
Example 7 describes the isolation of a full length human V28 cDNA.
Example 8 sets out a description of a full length human V112 cDNA.
Example 9 describes the isolation of a full length genomic DNA encoding human R20.
The isolation of full length R2 and R12 7TM receptor genes from a human genomic fetal liver library is detailed in Example 10.
Example 11 describes the cloning of a cDNA encoding the RM3 7TM receptor.
Example 12 presents a comparison of the amino acid sequences of 7TM receptors of the invention with amino acid sequences of previously described 7TM receptors.
The transfection of human cells with genomic and cDNA sequences encoding the 7TM receptor V31 and the phenotype of the transfected cells are detailed in Example 13.
Expression of 7TM receptors of invention in various human tissues and hematopoietic cell lines as assayed by Northern blot and in situ hybridization is described in Example 14.
Examples 15 and 16 respectively describe the expression of V31 and R20 genomic sequences as fusion proteins with Glutathione-S-Transferase in E.
coli, while Example 17 describes the expression of V31 and V28 cDNA sequences as fusion proteins with Glutathione-S-Transferase in E.
coli.
Example 18 describes the generation of polyclonal sera reactive with the V31 fusion proteins and V31 peptides useful for generating monoclonal and polyclonal antibodies specific for V31