 Method for providing a cell culture from a transgenic non-human mammal |
| We claim: 1. A method of providing a cell culture comprising (1) providing a transgenic non-human ... |
|
| Method of gene transfer into chickens and other avian species |
| A method is described for introducing foreign genes or foreign nucleic acid sequences into somatic ... |
|
| DNA vector with isolated CDNA gene encoding metallopanstimulin |
| The present invention is based upon the discovery of a new and heretofore unrecognized type of ... |
|
| Method for preparing immunoglobulin fragments |
| Pursuant to the present invention, there is provided an improved method for specific fragmentation ... |
|
| Morpholino-subunit combinatorial library and method |
| The invention includes, in one aspect, a combinatorial library of non-biological oligomers formed ... |
|
| Method of producing transgenic animals |
| What is claimed is: 1. A method for transferring DNA into fertilized eggs of a non-human mammal, ... |
|
| Humanized immunoglobulins |
| OF THE INVENTION In accordance with the present invention, novel means of designing humanized ... |
|
| Recombinant retroviruses |
| Briefly stated, the present invention provides recombinant retroviruses carrying a vector construct ... |
|
| Transgenic bovine |
| In accordance with the above objects, the invention includes transgenes for producing recombinant ... |
|
|
Method for producing highly reactive polyisobutenes
| Details |
Inventors: Auer, Heinz; Kanne, Ulrich; De Vos, Andre;
Assignee: BASF Aktiengesellschaft (Ludwigshafen, DE)
Primary Examiner: Teskin; Fred
Assistant Examiner:
Attorney, Agent or Firm: Novak Druce & Quigg, Voight; Jason D.
A process for the preparation of highly reactive polyisobutenes is described, in which isobutene is polymerized in the presence of a complex of boron trifluoride and at least one cocatalyst in a liquid organic phase at a reaction temperature of from -60 to -4° C. and water is added in two stages to the organic phase for catalyst deactivation and catalyst extraction. |
|
DETAILED DESCRIPTION We claim: 1.
A process for the preparation of highly reactive polyisobutenes, in which a) isobutene is polymerized in the presence of a complex of boron trifluoride and at least one cocatalyst in a liquid organic phase at a reaction temperature of from -60 to -4° C.
, b) the organic phase which is substantially at the reaction temperature is brought into intimate contact with a first portion of water whose temperature is at least 80° C.
above the reaction temperature, with formation of an emulsion which contains finely dispersed water droplets in the organic phase, c) a second portion of water is added to the emulsion with formation of a continuous, predominantly organic phase and a continuous, predominantly aqueous phase, and d) the organic phase is separated from the aqueous phase.
2.
A process as claimed in claim 1, in which the sum of the first and second portions of water is from 5 to 100% by weight, based on the weight of the organic phase, and the weight ratio of the first portion to the second portion is from 1:10 to 10:1.
3.
A process as claimed in claim 1, in which the emulsion obtained by bringing the organic phase into contact with the first portion of water has a mixing temperature of from +5 to +50° C.
4.
A process as claimed in claim 1, in which the cocatalyst used is a secondary alcohol of 3 to 20 carbon atoms.
5.
A process as claimed in claim 1, in which polymerization is effected in step a) until polyisobutene molecules having a number average molecular weight of from 1 800 to 5 000 are obtained.
6.
A process as claimed in claim 1, in which, in order to separate the organic phase from the aqueous phase, first the main amount of the aqueous phase is removed, the organic phase containing still dispersed water droplets is passed through an apparatus having coalescence-promoting internals and the coalesced aqueous phase is separated from the organic phase.
7.
A process as claimed in claim 6, in which the organic phase freed from the main amount of the aqueous phase is mixed with a third portion of water before the passage through the apparatus having coalescence-promoting internals
|
| Related patents |
|
|
Device and mixture for testing for immune responses to food
In accordance with the present invention the aforementioned needs are fulfilled by providing an inexpensive, simple and quick test for detecting an individual's Type I, I...
|
|
|
Compounds for immunotherapy of prostate cancer and methods for their use
OF THE INVENTION As noted above, the present invention is generally directed to compositions and methods for the immunotherapy of prostate cancer. The inventive ...
|
|
|
Compositions and methods for the therapy and diagnosis of prostate cancer
Briefly stated, the present invention provides compositions and methods for the diagnosis and therapy of cancer, such as prostate cancer. In one aspect, the present ...
|
|
|
Superantigen based methods and compositions for treatment of diseases
What is claimed is: 1. A pharmaceutical compound comprising a therapeutic antigen and superantigen, wherein said super antigen is conjugated to the therapeutic antigen ...
|
|
|
Tetra and penta-peptides useful in regulating the immune system
The present invention describes a series of thysplenin peptide analogs capable of regulating the function of cells of the immune system. As one aspect, the present ...
|
|
|
Peptide fragments of myelin basic protein
OF THE INVENTION All patent applications, patents and publications cited in this specification are hereby incorporated by reference in their entirety. In the case of ...
|
|
|
Targeted therapeutic delivery of vitamin D compounds
The present invention provides conjugates of vitamin D compounds or analogs and a targeting molecule, having an ability for site-specific delivery of the vitamin D. S...
|
|
|
Production and use of monoclonal antibodies to phosphotyrosine-containing proteins
OF THE PREFERRED EMBODIMENTS A hybridoma secreting monoclonal antibodies that bind to PT-residues of PT-proteins was isolated from a fusion of Sp 2/0 cells with spleen ...
|
|
|
Trichothecene antibody conjugates
I claim: 1. A conjugate of a trichothecene and an antibody or antibody fragment, said antibody or antibody fragment being capable of specifically binding to a defined ...
|
|
|
Monoclonal antibody to human protein C
What is claimed is: 1. A monoclonal antibody which is specific to human protein C which has calcium bound at the gamma-carboxyglutamic acid (Gla) domain and does not ...
|
|
|
Drugs 
