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Method-for-producing-yeast-expressed-HPV-types-6-and-16-capsid-proteins

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Method for producing yeast expressed HPV types 6 and 16 capsid proteins

Details

Inventors: Buonamassa, Daniela Tornese; Greer, Catherine E.; Galeotti, Cesira L.; Bensi, Giuliano; Petracca, Roberto;
Assignee: Chiron Corporation (Emeryville, CA)
Primary Examiner: Salimi; Ali R.
Assistant Examiner:
Attorney, Agent or Firm: Hale; Rececca M. Robins; Roberta L. Harbin; Alisa A.

Mosaic VLPs of viral capsid proteins from different virus types are described, as are methods of making the same. Specifically, a diploid yeast strain that coexpresses the L1 and L2 capsid proteins of both HPV-6 and HPV-16 as mosaic VLPs is described. The mosaic VLPs induced the production conformational antibodies against both L1 proteins upon administration to mice.

DETAILED DESCRIPTION What is claimed is: 1.
A virus-like particle (VLP) comprising a first late 1 (L1) capsid protein from human papilloma virus (HPV) type 6 and a second L1 capsid protein from HPV type 16.
2.
The VLP of claim 1, further comprising an L2 capsid protein.
3.
A composition comprising the VLP of claim 1.
4.
The composition of claim 3 wherein the VLP is purified for immunization.
5.
The composition of claim 4, wherein the VLP comprises an L2 capsid protein.
6.
The composition of claim 5, further comprising an adjuvant.
7.
The composition of claim 6 wherein the adjuvant is MF59.
8.
The VLP of claim 1 wherein said VLP induces an immune response against both types of viruses.
9.
A composition comprising the VLP of claim 8.
10.
A method for producing the VLP of claim 1, said method comprising a) cloning said capsid proteins into expression cassettes comprising the same promoters and termination sequences; and b) expressing said cassettes in the same yeast diploid host cell.
11.
The method of claim 10 wherein the yeast is Saccharomyces cerevisiae.
12.
The method of claim 10, wherein the capsid proteins further comprise L2 capsid proteins.
13.
The method of claim 12 wherein said L1 protein expression cassettes are cloned into non-integrative vectors, and said L2 proteins expression cassettes are cloned into integrative vectors.
14.
The method of claim 13 wherein the non-integrative vector is pBS24.
1.
15.
The method of claim 13 wherein the integrative vector is pUC8.
16.
A method for inducing an immune response against more than one type of virus comprising administering the VLP of claim 1.
17.
A method for inducing an immune response against more than one type of virus comprising administering the composition of claim 5.

Description:

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