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Transgenic non-human animals capable of producing heterologous antibodies

As has been discussed supra, it is desirable to produce human immunoglobulins that are reactive ...

Transgenic non-human animals for producing heterologous antibodies

As has been discussed supra, it is desirable to produce human immunoglobulins that are reactive ...

Nucleic acids encoding lymphoid CD30 antigen

We claim: 1. An isolated nucleic acid molecule comprising a polynucleotide having a sequence ...

Feeding tray for multiwell test apparatus

This invention will be described herein with reference to the growing and use of cells on a ...

Neo-tryptophan

What is claimed is: 1. A method of making a neurotensin polypeptide containing neo-tryptophan, said ...

Human immunodeficiency virus decoy

OF THE INVENTION The present invention has wide application to immunologic procedures and methods ...

Capsid forming and cystein modified chimaeric MS2-coat protein

We claim: 1. A modified coat protein capable of forming a capsid which comprises the coat protein ...

Polynucleotides related to monoclonal antibody 1A7 and use for the treatment of melanoma and small cell carcinoma

This invention relates to the discovery of an anti-idiotype antibody that is capable of recruiting ...

Strategically modified hepatitis B core proteins and their derivatives

The present invention relates to a strategically modified hepatitis B core (HBc) protein that is ...

Chimeric biotin-binding papillomavirus protein

OF THE INVENTION The first domain of the chimeric protein includes at least a portion of a ...

Methods and compositions useful in the diagnosis and treatment of autoimmune diseases

Details

Inventors: Keene, Jack D.;
Assignee: Duke University (Durham, NC)
Primary Examiner: Ziska; Suzanne E.
Assistant Examiner:
Attorney, Agent or Firm: Oblon, Spivak, McClelland, Maier & Neustadt, P.C.

A method for producing a protein antigen which is reactive with an autoantibody associated with an autoimmune disease in a host, which comprises introducing genetic information from a cross-reactive donor gene library, into plural cells thereby producing transformed cells: selecting a producer cell which expresses said antigen by detecting a binding reaction between said autoantibody obtained from said host and a protein antigen expressed by a producer cell of said transformed cells which contains a gene coding for said protein antigen, thereby identifying a cloned DNA segment from said donor which can be utilized in the production of said protein, is disclosed along with biochemical reagents and products associated with this invention.

DETAILED DESCRIPTION It is an object of this invention to provide a method for producing a protein antigen which is reactive with an autoantibody associated with an autoimmune disease.
It is a further object of this invention to provide a method for readily providing a protein antigen reactive with an autoantibody associated with an autoimmune disease.
It is a further object of this invention to provide a method capable of producing such antigens in large amounts and without requiring donation of large amounts of material from a host.
It is a further object of this invention to provide a method for readily providing high quantities of such a protein antigen without requiring donation of large amounts of material from a host.
It is a further object of this invention to provide a method for readily providing high quantities of such a protein antigen having a high degree of purity.
These and other objects of the invention as will hereinafter become more readily apparent have been accomplished by providing a method for production of a protein antigen which is reactive with an autoantibody associated with autoimmune disease in a host.
This method comprises introducing genetic information from a crossreactive donor gene library into plural recipient cells, thereby giving transformed cells.
A cell which expresses the antigen is selected by detecting a binding reaction between said autoantibody obtained from said host and a protein antigen expressed by a producer cell of said transformed cells which contains a gene coding for said protein antigen.
This cell which expresses the antigen is cultivated in a culture medium, and the protein antigen is isolated from the culture medium.
It is also an object of this invention to provide recombinant DNA molecules useful in the practice of the method of the invention as well as methods of using the protein antigens, now available for the first time in large quantities, for the diagnosis and treatment of autoimmune diseases.
It is also an object of this invention to provide the protein antigens obtained from the process of this invention

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