Process for preparation of a microbial agent

DETAILED DESCRIPTION I claim: 1.
A microbial control agent made by the process of: isolating Chilodonella uncinata; colonizing Chilodonella uncinata under laboratory conditions using artificial media; submerging said Chiodonella uncinata in sterile sand; allowing the Chilodonella uncinata and sterile sand to dry at room temperature; thereby producing a dry formulation of Chilodonella uncinata.
2.
The microbial agent of claim 1, wherein said Chilodonella uncinata are isolated from infected larvae and adult mosquitoes.
3.
The microbial agent of claim 1, wherein said dry formulation comprises inactive Chilodonella uncinata.
4.
A method of killing mosquitoes comprising administering the microbial agent of claim 1.
5.
The method of claim 4, wherein said microbial control agent is released in distilled water containing mosquito larvae under conditions such that inactive Chilodonella uncinata is reconstituted, reconstituted Chilodonella uncinata attacks and enters the larvae, and the Chilodonella uncinata kills the larvae and escapes the larvae.
6.
The method of claim 4, wherein said reconstituted Chilodonella uncinata enter the larvae through a cuticle by forming small holes in the cuticle.
7.
The method of claim 5, wherein the reconstituted Chilodonella uncinata enter the larvae through a haemocoelomic cavity of the larvae.
8.
The method of claim 5, wherein said Chilodonella uncinata which escapes from the dead larvae is cultivated at room temperature in the presence of artificial nutrition media to produce daughter cells, thereby continuing a cycle of infection.
9.
The method of claim 5, wherein said reconstituted Chilodonella uncinata remains active for at least two successive generations under lab laboratory conditions and is capable of being dispersed in the environment.
10.
A microbial control agent comprising isolated Chilodonella uncinata in a dry formulation of sterile sand which when released in water works as an effective biological agent for mosquito vectors of human disease