Process for preparing anti-obesity protein

DETAILED DESCRIPTION OF THE INVENTION For purposes of the present invention, as disclosed and claimed herein, the following terms and abbreviations are as defined below.
dDAP--a dipeptidylaminopeptidase, isolated from Dictyostelium discoideum, which demonstrates a pH optimum of about pH 3.
5 with GFpNA as a substrate and has a native molecular weight of about 225,000 daltons, as measured by analytical ultracentrifugation, and a subunit molecular weight of about 66,000 daltons, as measured by SDS polyacrylamide gel electrophoresis.
Support surface--any solid or semi-solid surface or matrix that can be used as is or easily derivatized or activated to bond a protein, exhibits minimal non-specific adsorption, is physically mechanically and chemically stable, is highly porous to provide ligand accessibility, and can be regenerated without deteriorating the surface.
dDAP bed--any amount of dDAP immobilized to a single or multiple support surface that forms an aggregate volume or unit of immobilized dDAP.
GFpNA--Gly-Phe p-nitroanilide.
SEQ ID NO: 1 refers to the sequence set forth in the sequence listing and means an anti-obesity protein of the formula: ##STR1## wherein: Xaa at position 4 is Gln or Glu; Xaa at position 7 is Gln or Glu; Xaa at position 22 is Gln, Asn, or Asp; Xaa at position 27 is Thr or Ala; Xaa at position 28 is Gln, Glu, or absent; Xaa at position 34 is Gln or Glu; Xaa at position 54 is Met, methionine sulfoxide, Leu, Ile, Val, Ala, or Gly; Xaa at position 56 is Gln or Glu; Xaa at position 62 is Gln or Glu; Xaa at position 63 is Gln or Glu; Xaa at position 68 is Met, methionine sulfoxide, Leu, Ile, Val, Ala, or Gly; Xaa at position 72 is Gln, Asn, or Asp; Xaa at position 75 is Gln or Glu; Xaa at position 78 is Gln, Asn, or Asp; Xaa at position 82 is Gln, Asn, or Asp; Xaa at position 100 is Gln, Trp, Tyr, Phe, Ile, Val, or Leu; Xaa at position 108 is Asp or Glu; Xaa at position 130 is Gln or Glu; Xaa at position 134 is Gln or Glu; Xaa at position 136 is Met, methionine sulfoxide, Leu, Ile, Val, Ala, or Gly; Xaa at position 138 is Gln, Trp, Tyr, Phe, Ile, Val, or Leu; and Xaa at position 139 is Gln or Glu