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Pseudomonas gladioli and a process for biologically controlling fusarium diseases using Pseudomonas gladioli pv. gladioli
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Inventors: Kijima, Toshio; Tezuka, Tokuya; Doi, Yoji; Yamashita, Shuiichi; Namba, Shigetoh; Arie, Tsutomu;
Assignee: Tochigi Prefecture (Tochigi Prefecture, JP)
Primary Examiner: Wax; Robert A.
Assistant Examiner:
Attorney, Agent or Firm: Wenderoth, Lind & Ponack
A new Pseudomonas gladioli having the identifying characteristics of Bikohken-kin No. 8805 has been discovered. The microorganism is a new bacteria separated from a bulb and roots of Miltonia. For separation, the bulb and roots of Miltonia are ground in a 1% solution of peptone followed by a streak culture on a bouillon agar at 25.degree. C. for 48-96 hours, and the colonies thus grown are isolated. This microorganism is inoculated into a bulb and roots of a plant selected from the group consisting of Welsh onion, sorgo, oats and maize. The plants inoculated with the grown microorganisms are grown together within the radius of rhizosphere of a plant to be protected (or companion or mixed crop) for further multiplication of Pseudomonas gladioli M-2196 in order to control soil borne plant diseases caused by Fusarium oxysporum. Very strong antibacterial activity on Fusarium oxysporum, Rhizoctonia solani, Verticillum dahliae, Corynebacterium michiganese pv. michiganese, Sclerotium cepivorum etc. is observed. |
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DETAILED DESCRIPTION What is claimed is: 1.
A biologically pure culture of the microorganism Pseudomonas gladioli for biologically controlling plant disease caused by Fusarium oxysporum, having the identifying characteristics of Bikohken-kin No.
8805, said culture having antifungal activity and also being capable of growing in a bulb and/or roots of at least a plant selected from the group consisting of Welsh onion, sorgo, oats and maize.
2.
A process for biologically controlling plant disease caused by Fusarium oxysporum using Pseudomonas gladioli pv.
gladioli, which comprises a step of inoculating Pseudomonas gladioli M-2196 into a bulb and/or roots of at least a plant selected from the group consisting of Welsh onion, sorgo, oats and maize; a step of planting the inoculated plants together within the radius of rhizosphere of a plant to be protected for further multiplication of Pseudomonas gladioli M-2196; and a step of controlling soil borne plant disease by means of antifungal activity against Fusarium oxysporum.
Description:
BACKGROUND OF INVENTION 1.
Field of Invention This invention relates to a new Pseudomonas gladioli and a process for biologically controlling Fusarium diseases caused by Fusarium oxysporum using Pseudomonas gladioli pv.
gladioli.
2.
Description of the Prior Art Soil borne plant diseases such as Fusarium wilt, Fusarium root rot etc.
are caused by Fusarium oxysporum, Rhizoctonia solani, Rhizoctonia rot, etc.
A soil disease is conventionally considered one of the malignant diseases, and once a plant is infected with the soil borne diseases, it is almost impossible to control it, usually causing annihilation of the neighboring plants as well.
Various searches for new soil microorganisms which have antibacterial activity on the aforementioned plant diseases have been made, but no soil microorganisms having strong antibacterial activity have been discovered.
Miltonia is widely cultivated in Tochigi Prefecture in Japan.
The present inventors discovered a new microorganism which belongs to Pseudomonas gladioli and exhibits strong antibacterial activity on Fusarium oxysporum, Rhizoctonia solani etc
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