Recombinant molecule encoding human protease nexin

DETAILED DESCRIPTION We claim: 1.
An isolated DNA molecule which encodes mature protease nexin-I (PN-I) alpha of FIG.
3 or PN-I beta of FIG.
4 or encodes a PN-I protein encoded by a gene which is a naturally occurring allelic variant of the gene encoding PN-I alpha or PN-I beta.
2.
The DNA molecule of claim 1 which further includes DNA encoding a signal sequence which is operably linked to said mature PN-1.
alpha.
or PN-1.
beta.
.
3.
The DNA molecule of claim 2 wherein the linked DNA encodes the signal sequence of mature human PN-I.
4.
A probe which is capable of hybridizing to mammalian cDNA that encodes PN-I and which is selected from the group consisting of (1) the Sau3AI fragment of PN-18; (2) the 750 bp EcoRI-Bg1II fragment of PN-18; (3) the 14-mer of FIG.
1a; and (4) the 36-mer of FIG.
1b.
5.
An expression vector comprising a DNA encoding a protease nexin I (PN-I) having the amino acid sequence of PN-I alpha of FIG.
3 or PN-I beta of FIG.
4, or having an amino acid sequence encoded by a gene which is a naturally occurring allelic variant of that encoding said PN-I alpha or PN-I beta wherein said expression vector is capable, when transformed into a host cell, of expressing said PN-I encoding DNA.
6.
The expression vector of claim 5 wherein the PN-I encoding DNA includes DNA encoding an operably linked signal sequence.
7.
A unicellular host cell transformed with the expression vector of claim 5.
8.
The host cell of claim 7 wherein said host cell is a mammalian cell and wherein said vector comprises an SV40-derived promoter.
9.
A method of producing PN-I alpha or PN-I beta which comprises culturing cells transformed with the expression system of claim 5 under conditions which induce the expression of said PN-I encoding DNA and recovering the PN-I protein produced.




Description:
TECHNICAL FIELD The invention relates to recombinant production of proteins affecting the cardiovascular system.
In particular, it concerns the cloning and expression of genes encoding forms of protease nexin I (PN-I)