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| Trichothecene antibody conjugates |
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| Monoclonal antibody to human protein C |
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| Method for providing a cell culture from a transgenic non-human mammal |
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| Method of gene transfer into chickens and other avian species |
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| DNA vector with isolated CDNA gene encoding metallopanstimulin |
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| Method for preparing immunoglobulin fragments |
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| Morpholino-subunit combinatorial library and method |
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| Method of producing transgenic animals |
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| Humanized immunoglobulins |
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Single polypeptide chain binding molecules
| Details |
Inventors: Ladner, Robert C.; Bird, Robert E.; Hardman, Karl;
Assignee: Enzon, Inc. (South Plainfield, NJ)
Primary Examiner: Lacey; David L.
Assistant Examiner: Budens; Robert D.
Attorney, Agent or Firm: Goldstein; Jorge A.
The invention pertains to a single polypeptide chain binding molecule which has binding specificity and affinity substantially similar to the binding specificity and affinity of the light and heavy chain aggregate variable region of an antibody, to genetic sequences coding therefor, and to recombinant DNA methods of producing such molecule and uses for such molecule. |
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DETAILED DESCRIPTION The present invention starts with a computer based system and method to determine chemical structures for converting two naturally aggregated but chemically separated light and heavy polypeptide chains from an antibody variable region into a single polypeptide chain which will fold into a three dimensional structure very similar to the original structure made of the two polypeptide chains.
The single polypeptide chain obtained from this method can then be used to prepare a genetic sequence coding therefor.
The genetic sequence can then be replicated in appropriate hosts, further linked to control regions, and transformed into expression hosts, wherein it can be expressed.
The resulting single polypeptide chain binding protein, upon refolding, has the binding characteristics of the aggregate of the original two (heavy and light) polypeptide chains of the variable region of the antibody.
The invention therefore comprises: A single polypeptide chain binding molecule which has binding specificity substantially similar to the binding specificity of the light and heavy chain aggregate variable region of an antibody.
The invention also comprises genetic sequences coding for the above mentioned single polypeptide chain, cloning and expression vectors containing such genetic sequences, hosts transformed with such vectors, and methods of production of such polypeptides by expression of the underlying genetic sequences in such hosts.
The invention also extends to uses for the binding proteins, including uses in diagnostics, therapy, in vivo and in vitro imaging, purifications, and biosensors.
The invention also extends to the single chain binding molecules in immobilized form, or in detectably labelled forms for utilization in the above mentioned diagnostic, imaging, purification or biosensor applications.
It also extends to conjugates of the single polypeptide chain binding molecules with therapeutic agents such as drugs or specific toxins, for delivery to a specific site in an animal, such as a human patient
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