Culturable mitochondrial cells with no nucleus, showing mitochondrial activity

DETAILED DESCRIPTION OF THE INVENTION Hereinafter, the present invention will be described in detail.
(1) Method of Producing the Cells of the Present Invention The present invention relates to a method of producing a culturable cell with no nucleus, showing mitochondrial activity, comprising: performing cell fusion between a nucleus-less cell having mitochondrial DNA and a mitochondrial DNA-less cultured cell derived from a cancer cell; culturing resulting cybrid cells; and recovering floating cells from obtained cultured cells.
Hereinafter, a culturable cell with no nucleus, showing mitochondrial activity is also referred to as a "mitochondrial cell.
" The nucleus-less cell having mitochondrial DNA used in the method of the present invention may be a cell that is produced by enucleating a cell that has a nucleus and mitochondrial DNA or a cell that genetically lacks a nucleus but has mitochondrial DNA.
It is preferred that the cell that genetically lacks a nucleus but has mitochondrial DNA be used.
Examples of such a cell include platelets.
The cell that lacks a nucleus but has mitochondrial DNA can be obtained from an organism that has mitochondrial DNA in the cell.
As such an organism, mammals are preferred with human being particularly preferred.
In the method of the present invention, use of cells obtained from patients with mitochondria-associated diseases from whom informed consents were obtained in advance as the cells that lack a nucleus but have mitochondrial DNA can provide mitochondrial cells each having a mutation of mitochondrial disease.
Examples of mitochondria-associated diseases include mitochondrial diseases such as Leigh syndrome, Alzheimer's disease and Parkinson's disease.
The mtDNA-less cultured cells derived from cancer cells that are used in the method of the present invention include known Rho.
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0 cell lines that have a nucleus but no mtDNA.
Specific examples thereof include Hela Rho.
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0 cell line, fibroblast Rho.
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0 cell line, and osteosarcoma Rho.
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0 cell line