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Electroblotting technique for transferring specimens from a polyacrylamide electrophoresis or like gel onto a membrane
| Details |
Inventors: Littlehales, William J.;
Assignee: American Bionetics, Inc. (Hayward, CA)
Primary Examiner: Niebling; John F.
Assistant Examiner: Starsiak, Jr.; John S.
Attorney, Agent or Firm: Fulwider, Patton, Rieber, Lee & Utecht
An apparatus especially suitable for transferring a particular specimen from a polyacrylamide electrophoresis or like gel onto a support sheet by means of electroblotting is disclosed herein along with its method of operation. This apparatus utilizes a pair of plate-shaped electrode assemblies, each of which in the disclosed embodiment is a multilayered assembly including an electrode layer of electrically conductive material and a layer of thermally conductive material which serves as a heat sink. These electrode assemblies are adjustably supported in spaced-apart but confronting relationship to one another with the gel and support sheet sandwiched therebetween. At the same time, the electrode layers are connected to a source of power for producing an electric field across the gel and support sheet in order to transfer the specimen from the gel to the sheet by means of electroblotting. In the specific embodiment disclosed, the spacing between the electrode assemblies can be adjustably decreased at a number of different points in order to ensure that they remain parallel to one another in their adjusted positions whereby to squeeze the gel and support sheet uniformly along the extend of the gel. |
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DETAILED DESCRIPTION What is claimed is: 1.
An apparatus especially suitable for transferring a biological specimen from a polyacrylamide electrophoresis or like gel onto a support sheet by means of electroblotting, said apparatus comprising: (a) a press arrangement including a pair of plate-shaped electrode assemblies having facing layers of electrically conductive material, means for maintaining said electrode assemblies in a confronting, generally parallel relationship with one another and adapted to accommodate said gel and transfer sheet sandwiched therebetween, and means for adjustably decreasing the spacing between said electrode assemblies at a plurality of different points to squeeze the gel and transfer sheet together substantially uniformly along the entire extent of the gel; and (b) means for connecting said electrode assemblies to a source of power for producing an electric field across said gel and support sheet in order to transfer said specimen from the gel to the sheet by means of electroblotting.
2.
An apparatus according to claim 1 wherein each of said electrode assemblies is a multilayered assembly including a backing layer of aluminum which serves as a heat sink.
3.
An apparatus according to claim 1 wherein the electrically conductive facing layer of each electrode assembly is formed from an electrically conductive, carbon loaded polymeric material.
4.
An apparatus according to claim 1 wherein said means for supporting said electrode assemblies includes a pair of confronting support plates with said electrode assemblies disposed between and mounted to respective ones of said plates.
5.
An apparatus according to claim 4 wherein said means for supporting said electrode assemblies also includes a plurality of threaded support rods extending between said support plates at various points across the plates and wherein said means for adjusting the spacing between electrode assemblies includes an adjustment knob carried by each of said support rods.
6.
An apparatus according to claim 5 wherein said adjustment knobs include substantially identical loadsprings, each loadspring extending concentrically around an associated rod with one end engaged against its knob and an opposite end engaged against one of said support plates
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