Electroporation device

DETAILED DESCRIPTION I claim: 1.
An electroporation apparatus for electroporating living cells with molecules comprising: (a) first and second electrodes, respectively having, first and second mutually opposed electrode surfaces, said first and second mutually opposed electrode surfaces being positioned to create an electric field there-between when connected to a source of electrical potential, said first electrode being only partially conductive and exhibiting a voltage drop across its said first electrode surface upon the passage of current therethrough; (b) an electrolyte positioned between said first and second said electrolyte containing molecules to be deposited within living cells by electroporation and being in contact with said first and second electrode surfaces; (c) living cells attached to said first electrode surface and being in contact with said electrolyte, and (d) containment means positioned to contain the electrolyte and living cells between said first and second electrodes, wherein by reason of the spacing between opposed portions of said first and second mutually opposed electrode surfaces, said second electrode, in combination with said first electrode, provides a local electrical field over said first electrode surface which is of substantially equal strength over said first electrode surface.
2.
An apparatus as in claim 1 wherein said first and second mutually opposed electrode surfaces are planar.
3.
An apparatus as in claim 2, wherein said second electrode surface is angled with respect to said first electrode surface to create along said first electrode surface said local electrical field of substantially equal strength.
4.
An apparatus as in claim 1, 2 or 3 wherein; (a) said first electrode comprises a non-conductive substrate; (b) said first electrode surface comprises a partially conductive layer bonded to said non-conductive substrate, and (c) said non-conductive substrate and partially conductive layer are sufficiently transparent or translucent to allow viewing of the living cells on said first electrode surface under a microscope by transmitted light