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| I claim: 1. An electroporation apparatus for electroporating living cells with molecules comprising:... |
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| Implantable electroporation method and apparatus for drug and gene delivery |
| Accordingly, it is the primary object of the present invention to provide an improved method of ... |
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| Flow electroporation chamber with electrodes having a crystalline metal nitride coating |
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| Solely pollen-specific promoter |
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| Gene transfer using transformed, neodetermined, embryonic cells |
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| Treatment to prevent loss of and/or increase bone mass in metabolic bone diseases |
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| Imidazole-containing cationic amphiphiles for intracellular delivery of therapeutic molecules |
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| Cationic amphiphiles containing multiplesteroid lipophilic groups |
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| N-terminally chemically modified protein compositions and methods |
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Injectable hyaluronic acid derivative with pharmaceuticals/cells
| Details |
Inventors: Radice, Marco; Pastorello, Andrea; Pavesio, Alessandra; Callergaro, Lanfranco;
Assignee: Fidia Advanced Biopolymers, SRL. (Abano Terme, IT)
Primary Examiner: Tsang; Cecilia J.
Assistant Examiner: Hanely; Susan
Attorney, Agent or Firm: Birch, Stewart, Kolasch & Birch, LLP
Disclosed is an injectable, biocompatible and biodegradable composition, comprising at least one hyaluronic acid benzyl ester or auto-cross-linked derivative, in combination with at least one mammalian cell and/or at least one pharmacologically or biologically active substance and/or micro-particles such as fibres, granules, microspheres or sponge fragments of a hyaluronic acid derivative. |
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DETAILED DESCRIPTION OF THE INVENTION The present invention concerns injectable, biocompatible and biodegradable compositions containing at least one hyaluronic acid benzyl ester derivative and/or auto-crosslinked derivative, at least one pharmacologically and/or biologically active substance, such as a growth factor, and/or at least one mammalian cell, particularly chondrogenic cells.
1.
The Hyaluronic Acid Component The present invention, therefore, describes injectable biocompatible compositions based on a benzyl ester of hyaluronic acid or on an auto-cross-linked derivative of hyaluronic acid, used singly or in mixtures with one another, characterized by high biocompatibility.
The materials are also completely biodegradable and do not need to be removed from the application site, thus avoiding a second surgical operation.
When prepared in the form of gels, the cross-linked derivatives present materials with significantly greater viscosity than the unmodified polymer and with variable degradation times.
The term "hyaluronic acid" is used in literature to designate an acidic polysaccharide with various molecular weights constituted by resides of D-glucuronic acid and N-acetyl-D-glucosamine, which naturally occur in cellular surfaces, in the basic extracellular substances of the connective tissues of vertebrates, in the synovial fluid of joints, in the vitreous humor of the eye, in the tissue of the human umbilical cord and in cocks' comb.
Hyaluronic acid plays an important role in the biological organism, firstly as a mechanical support of the cells of many tissues, such as the skin, the tendons, the muscles and cartilage and it is therefore the main component of the extracellular matrix.
But hyaluronic acid also performs other functions in the biological processes, such as the hydration of tissues, lubrication, cellular migration, cell function and differentiation.
(See for example, A.
Balazs et al.
, Cosmetics & Toiletries, No.
5/84, pages 8 17).
Hyaluronic acid may be extracted from the above-mentioned natural tissues, such as cocks' combs, or also from certain bacteria
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Gene Therapy 
