L-654,040, antibacterial agent

DETAILED DESCRIPTION OF THE INVENTION Antibacterial L-654,040 is obtained by growing under controlled conditions the microorganism Streptoverticillium synroense MA6011, ATCC 53699 in a fermentation broth.
The fermentation may be carried out in media containing suspended nutrient matter or in predominantly clear media wherein the medium is substantially free of suspended nutrient matter.
Based on extensive taxonomic studies, the antibiotic producing microorganism is identified as the new species Streptoverticillium synroense.
A useful strain is designated MA6011 in the culture collection of MERCK & CO.
, Inc.
, Rahway, N.
J.
On or about Dec.
10, 1987, a culture thereof has been placed on permanent deposit with the culture collection of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md.
20852, and has been assigned Accession No.
ATCC 53699.
MORPHOLOGICAL AND CULTURAL CHARACTERISTICS OF STREPTOVERTICILLIIUM SYNROENSE MA6011, ATCC 53699 The morphological and cultural characteristics of Streptoverticillium synroense MA6011, ATCC 53699 are set forth below.
Morphology Using standard media and procedures, morphological and cultural characteristics of the producing organism were examined according to Bergey, Manual of Determinative Bacteriology, 8th Edition, Williams & Wilkins.
Based on comparison with the published description of other species of the genus Streptoverticillium, it was determined that the producing organism is a new strain of a new species, identified in the Merck collection as Streptoverticillium synroense, strain MA6011.
CULTURAL CHARACTERISTICS OF STREPTOVERTICILLIUM SYNROENSE MA-6011, PRODUCING ORGANISM OF L-654,040 A.
Morphology Sporophores form short branches produced in a verticil or whorl at intervals along the aerial mycelia.
These branches in turn produce several secondary branches that form straight chains of 10-15 spores.
Spore surface appears rough, almost warty at high magnifications (EM).
Yeast Extract-Malt Extract Agar (ISP Medium 2) V.
sup.
1 : Reverse - black A: Mixed light and medium gray SP: Very dark brown