Labelled oligonucleotides synthesized on solid-supports

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Reference will now be made in detail to the preferred embodiments of the invention, examples of which are illustrated in the accompanying drawings.
While the invention will be described in conjunction with the preferred embodiments, it will be understood that they are not intended to limit the invention to those embodiments.
On the contrary, the invention is intended to cover alternatives, modifications, and equivalents, which may be included within the invention as defined by the appended claims.
I.
Definitions Unless stated otherwise, the following terms and phrases as used herein are intended to have the following meanings: The terms "nucleic acid", "polynucleotide" or "oligonucleotide" mean polymers of nucleotide monomers or analogs thereof, including double and single stranded deoxyribonucleotides, ribonucleotides, .
alpha.
-anomeric forms thereof, and the like.
The oligonucleotide may comprise one or more DNA, RNA, and nucleic acid analog monomer units.
The monomers are linked by internucleotide linkages, including phosphodiester bonds or phosphate analogs thereof, and associated counterions, e.
g.
, H.
sup.
+, NH.
sub.
4.
sup.
+, Na.
sup.
+.
Oligonucleotides typically range in size from a few monomeric units, e.
g.
5-40, to several thousands of monomeric units.
Whenever an oligonucleotide is represented by a sequence of letters, such as "ATGCCTG," it will be understood that the nucleotides are in 5' to 3' order from left to right and that "A" denotes deoxyadenosine, "C" denotes deoxycytidine, "G" denotes deoxyguanosine, and "T" denotes thymidine, unless otherwise noted.
"Nucleoside" refers to a compound consisting of a purine, deazapurine, or pyrimidine nucleobase, e.
g.
, adenine, guanine, cytosine, uracil, thymine, deazaadenine, deazaguanosine, and the like, linked to a pentose at the 1'-position.
When the nucleoside base is purine or 7-deazapurine, the pentose is attached to the nucleobase at the 9-position of the purine or deazapurine, and when the nucleobase is pyrimidine, the pentose is attached to the nucleobase at the 1-position of the pyrimidine