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| Tricyclic macrolide antibacterial compounds |
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| Gene encoding MNR2 and uses thereof |
| OF THE INVENTION The following standard abbreviations are used throughout the specification to ... |
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| Polyene microlide pre-liposomal powders |
| OF THE PREFERRED EMBODIMENT A stable powder suitable for the direct preparation of liposome-... |
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| Process for stabilizing liposomes |
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| cDNA encoding human phospholipase A.sub.2 polypeptide |
| OF THE INVENTION The present invention provides a novel intracellular human phospholipase A.sub.2 ... |
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| DNA encoding Bcl-2-associated proteins |
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| Hybridoma-producing NSO myeloma cell line |
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Method and marker for identification of pre-malignancy and malignancy and therapeutic intervention
| Details |
Inventors: Mai, Sabine;
Assignee: University of Manitoba (Winnipeg, CA); Cancercare Manitoba (Winnipeg, CA)
Primary Examiner: Myers; Carla J.
Assistant Examiner:
Attorney, Agent or Firm: Kohn & Associates, PLLC, Kohn; Kenneth I.
There is provided a method for identifying pre-malignancy, malignancy, and degree of pre-malignancy and malignancy of a cell by detecting extrachromosomal and intrachromosomal gene amplification. Also provided is a marker for the identification of pre-malignancy, malignancy, and degree of pre-malignancy and malignancy of a cell containing extrachromosomal and intrachromosomal gene amplification of a gene. A diagnostic tool for the diagnosis and prognosis or cervical cancer containing extrachromosomal and intrachromosomal gene amplification of a gene. |
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DETAILED DESCRIPTION OF THE INVENTION The present invention provides a method of identifying pre-malignant and malignant cells in cells and tissues.
For example the present invention allows identification of micrometastasis, plasmacytomas, cervical cancer, head and neck cancer and CLL and other B-cell leukemias.
More specifically, the method includes detecting the presence of extrachromosomal gene amplification in a cell.
Additionally, a marker is disclosed which is used in the above method for identifying pre-malignancy and malignancy states in a cell by determining if extrachromosomal gene amplification of the marker is present.
The method of the present invention uses as a marker the presence of extrachromosomal gene amplification as the marker.
Further, the present invention has unexpectedly determined that the specific genes that are amplified extrachromosomally are involved in initiation of tumorigenesis and therefore provide therapeutic targets.
Therapeutic treatment including gene therapy as for example utilizing suicide genes targeted to the extrachromosomal elements or antisense therapy targeted to the identified genes can be utilized.
By extrachromosomal, it is meant a factor which exists, at least for a time, independent of the chromosome.
Accordingly, the extrachromosomal factor is not a part of the chromosome, however these factors can be considered a genetic unit fully equal to those in the chromosomes.
Standard molecular biology techniques known in the art and not specifically described are generally followed as in Sambrook et al.
, Molecular Cloning: A Laboratory Manual, Cold Springs Harbor Laboratory, New York (1989, 1992), and in Ausubel et al.
, Current Protocols in Molecular Biology, John Wiley and Sons, Baltimore, Md.
(1989).
Additionally, standard methods in immunology known in the art and not specifically described are generally followed as in Stites et al.
(eds), Basic and Clinical Immunology (8th Edition), Appleton & Lange, Norwalk, Conn.
(1994) and Mishell and Shiigi (eds), Selected Methods in Cellular Immunology, W
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Gene Therapy 
