DETAILED DESCRIPTION OF THE INVENTION In the context of the present invention the following definitions apply: Incubation: the introduction and leaving of a biological object, such as an enzyme, NOS, in a medium to which, possibly, further compounds are or have been added, such as the enzyme substrate.
For this incubation appropriate (usually similar to physiological) conditions are chosen, in order to achieve a certain effect.
For example, an enzyme reaction takes place, e.
g.
, the conversion of arginine into citrulline and NO catalyzed by NOS.
The conditions are achieved, for example, by controlling the temperature, pH, coenzymes or cofactors and/or reaction time etc.
Reaction vessel: a vessel in which a chemical reaction, or a separation based on bonding or other physical forces, can take its course under controlled conditions.
Examples include an Eppendorf cup, a culture dish or flask, a test tube, a centrifuge tube or a well of a microtiter plate.
A reaction vessel may be divided into compartments by selectively permeable membranes.
It does not necessarily have to be sealed so that fluids cannot pass through, not even on the underside of the vessel.
Thus reaction vessels can be columns or microtiter plates also sealed on one side (usually the underside) by a selectively permeable membrane, such as a filter-plate membrane.
The incubation (step a), and preferably also the separation (step b), takes place in a reaction vessel.
Separation: by this the spatial separation of arginine from citrulline is understood, so that, unlike the incubation mixture which contains both substances homogeneously in a certain proportion, separate areas are formed so that the arginine is almost completely confined in one area and the citrulline has almost been completely removed in comparison to the previous concentration, and in an other area the opposite is the case.
The separation can, for example, be effected physically by means of size or molecular weight filters, by means of charging (cation exchangers, chromatographically or by applying voltage), or, for example, by centrifugation
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Gene Therapy 
