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Home Gene Therapy Methods-and-compositions-for-expression-of-competent-eukaryotic-gene-products

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 Methods and compositions for expression of competent eukaryotic gene products

Details
Inventors: Bromley, Peter; Voellmy, Richard;
Assignee: Rockwell Property Limited (Isle of Man, GB)
Primary Examiner: Low; Christopher S. F.
Assistant Examiner:
Attorney, Agent or Firm: Cushman Darby & Cushman Intellectual Property Group of Pillsbury Madison & Sutro LLP

Heat-shock gene control elements from different eukaryotic cells are utilized to provide expression of competent gene products in the same or similar eukaryotic as well as in procaryotic host cells. The control elements can be joined to a suitable eukaryotic replication system to form an expression vector, or may be joined to the gene of interest and introduced directly into the host genome.

DETAILED DESCRIPTION DNA constructions are provided comprising control elements derived from heat-shock genes associated with genes of interest, which permit the expression of the gene of interest in both procaryotic and eucaryotic cells.
In particular, for the efficient synthesis of fully competent gene products, a homologous combination of expression unit and host cell for expression is used and the nature of this homologous system is that of the same or similar cell type or organism that normally produces the said gene product.



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