Modified polypeptides with high activity and reduced allergenicity

DETAILED DESCRIPTION OF THE INVENTION The modified polypeptide of the present invention is represented by the formula: A-B.
sub.
n containing, as essential components, a enzyme, A, and a plurality, n, of twin polymer moieties, B.
While not intending to be limited by theory, it is believed that the conjugation of the twin polymer moieties to the enzyme provides a balanced stearic hindrance of the activated surface of the enzyme as to allow for high activity but simultaneously prevent stimulation of the immune system and subsequent antibody formation responsible for allergic reaction.
As used herein, the phrase "amino acid sequence" refers to a specific configuration of the amino acids comprising a polypeptide.
The following is a list of abbreviations used herein to describe amino acids:
Three-letter
Amino Acid Abbreviation One-letter Symbol
Alanine Ala A
Arginine Arg R
Asparagine Asn N
Aspartic Acid Asp D
Cysteine Cys C
Glutamine Gln Q
Glutamic Acid Glu Q
Glycine Gly G
Histidine His H
Isoleucine Ile I
Leucine Leu L
Lysine Lys K
Methionine Met M
Phenylalanine Phe F
Proline Pro P
Serine Ser S
Threonine Thr T
Tryptophan Trp W
Tyrosine Tyr Y
Valine Val V
No amino acid at position Xaa *
As used herein, the term "mutation" refers to the genetic alteration of an organism, which in turn alters the amino acid sequence of the enzyme produced by that organism