 Probe for electrofusion, electroporation, or like procedure |
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| Device and method for the electrophoretic separation of macromolecules |
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| Superfusion apparatus |
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| Electroporation device |
| I claim: 1. An electroporation apparatus for electroporating living cells with molecules comprising:... |
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| Implantable electroporation method and apparatus for drug and gene delivery |
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| Solely pollen-specific promoter |
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| Gene transfer using transformed, neodetermined, embryonic cells |
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Mutants of the RB and P53 genes
| Details |
Inventors: Fung, Yuen Kai;
Assignee: Research Development Foundation (Carson City, NV)
Primary Examiner: Kemmerer; Elizabeth
Assistant Examiner:
Attorney, Agent or Firm: Adler; Benjamin Aaron
The present invention provides mutants of the Rb and p53 genes and methods utilizing these mutants therapeutically. Along with mutated Rb genes and p53 genes, the present invention provides plasmids containing a mutated Rb gene or a p53 gene. In addition, the present invention provides cells transfected with the plasmids of the present invention. Moreover, the present invention provides for methods of treating a variety of pathophysiological cell proliferative diseases. |
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DETAILED DESCRIPTION OF THE INVENTION Definitions The term "functional expression of the gene" meant to include the suppression of transcription of the gene, the degradation of the gene transcript (pre-message RNA), the inhibition of splicing, the destruction of the message RNA, the prevention of translation of the message RNA, the prevention of the post-translational modifications of the protein, the destruction of the protein, or the inhibition of the normal function of the protein.
The term "transfected plasmid" is meant to include the bacterial plasmid which contains a mutated retinoblastoma or p53 gene to be carried (transfected) into the cell of choice.
The term"gene therapy" is meant to include the insertion of part or all of a gene, a DNA construct, RNA, or gene product into a cell, group of cells, tissue, pathologic lesion, organ or organism for the purpose of modulating gene expression, and/or function of the gene product.
The term "prophylactic gene therapy" is meant to include genes which may be used for partial or total inhibition or prevention of disease and the spread of disease and also is meant to include genes which may be used to supplement or replace absent or defective negative growth in cell, tissues or germlines.
The term "cell proliferative disease" is meant to include any human or animal disease or disorder, affecting any one or any combination of organs, cavities or body parts, which is characterized by single or multiple local abnormal proliferations of cells, groups of cells or tissue(s), whether benign or malignant.
The term "prokaryote" is meant to include all bacteria which can be transformed with the DNA for the expression of the recombinant molecules of the present invention.
The term "eukaryote" is meant to include all yeasts, fungi, animal and plant cells which can be transformed with the DNA for the expression of the recombinant molecules of the present invention.
The DNA for DNA constructs of the present invention can be synthetic or may be derived from any mammalian species
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