Nucleic acids encoding calcitonin receptor and uses thereof

DETAILED DESCRIPTION The Drawings The drawings are first described.
FIGS.
1A and 1B are a composite of graphs depicting binding of porcine calcitonin to cells.
FIG.
1A) Porcine calcitonin binding to LLC-PK.
sub.
1 cells; FIG.
1B) Porcine calcitonin binding to COS cells transfected with cDNA encoding porcine CTR.
Insets show Scatchard analysis of the binding data.
FIG.
2A is a histogram of the cAMP response in FIG.
2A) porcine calcitonin receptor-transfected COS cells and FIG.
2B) is a histogram of the cAMP response in COS cells mock-transfected with .
beta.
-galactosidase.
SCT=salmon calcitonin and ISO=isoproterenol.
FIG.
3 is the amino acid sequence of porcine CTR (SEQ ID NO:1) aligned with the PTH-PTHrP receptor.
The alignment was generated with UWGCG program GAP (Devereux et al.
, 1984, Nucl.
Acids Res.
12:387).
Shaded boxes represent identity or similarity.
The bars above the sequence represent the transmembrane domains.
Symbol # indicates N-linked glycosylation sites and + indicates conserved cysteines.
FIG.
4A (low power) and FIG.
4B (high power) are emulsion autoradiographs of BIN-67 cells in culture following incubation with [.
sup.
125 I]-salmon calcitonin.
FIG.
5A is a graph of binding of salmon calcitonin to BIN-67 cells, and FIG.
5B is a graph of binding of salmon calcitonin to COS cells transfected with the human CTR cDNA.
Insets show Scatchard analysis of binding data.
FIGS.
6A, 6B, and 6C are a composite of graphs of binding and cAMP dose response of human and salmon calcitonins in BIN-67 cells.
FIG.
6A) cAMP dose response curves to salmon and human calcitonin; FIG.
6B) Competition dissociation binding curves for [.
sup.
125 I]-human calcitonin competed with unlabeled human calcitonin; FIG.
6C) Human calcitonin binding to BIN-67 cells.
Maximal binding was 1.
92.
times.
10.
sup.
4 cpm per sample; the inset shows a Scatchard analysis of binding data.
FIGS.
7A, 7B, and 7C are histograms representing the cAMP response in BIN-67 cells and in transfected COS cells.
FIG.
7A) Mock (.
beta.
-galactosidase) transfected COS cells; FIG