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Nucleic acids encoding transcription factor APRF (acute phase response factor)
| Details |
Inventors: Kishimoto, Tadamitsu; Akira, Shizuo;
Assignee: Kishimoto; Tadamitsu (Osaka, JP)
Primary Examiner: Robinson; Douglas W.
Assistant Examiner: Wai; Thanda
Attorney, Agent or Firm: Sughrue, Mion, Zinn, Macpeak & Seas, PLLC
Mammal transcription factor APRF, process for the preparation of it, DNAs encoding the product, replication and expression vector comprising the DNA, host cells transformed or tranfected with the replication and expression vector, evaluating and screening method for searching an inhibitory agent on the function of APRF and an inhibitory agent against the function of the APRF. The peptide of the present invention (APRF) may be useful for complement or suppression of the function of APRF, screening an inhibitory agent against the function of APRF. Inhibiting agent containing the product as active ingredient of the present invention may be also useful for treatment of diseases related to cytokine such as IL-6, i.e., inflammatory diseases. |
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DETAILED DESCRIPTION What is claimed: 1.
An isolated DNA molecule which encodes a human APRF polypeptide having the amino acid sequence of SEQ ID No:1.
2.
An isolated DNA molecule comprising the nucleotide sequence of SEQ ID NO:2.
3.
An isolated DNA molecule comprising the nucleotide sequence of SEQ ID NO:3.
4.
A replication or expression vector comprising the DNA molecule of claim 1, 2 or 3.
5.
A host cell transformed or transfected in vitro with the replication or expression vector of claim 4.
6.
A method of producing mammalian acute phase response factor (APRF) having the amino acid sequence of SEQ ID NO:1 comprising culturing a host cell transformed or transfected with the replication or expression vector of claim 4.
7.
The host cell of claim 5 which is an Escherichia coli, a COS cell, a CHO cell or a mouse L cell.
8.
The method of claim 6, wherein said host cell is an Escherichia coli, a COS cell, a CHO cell or mouse L cell.
Description:
FIELD OF THE INVENTION The present invention is related to a novel transcription factor, acute phase response factor (abbreviated APRF hereafter), DNA's encoding it, evaluating and screening methods for searching an inhibitory agent against the function of APRF and an inhibitory agent against the function of the APRF.
More particularly, the present invention is related to APRF which is the transcription factor related to signal transmission of interleukin 6 (abbreviated as IL-6 hereafter) in cells, process for the preparation, DNA's encoding it, replication and expression vector comprising the DNA, host cells transformed or transfected with the replication and expression vector, evaluating and screening method for searching an inhibitory agent against the function of APRF and an inhibitory agent against the function of the APRF.
RELATED ARTS AND PROBLEMS Bio-signal transmitter mediates the response of cells by transmitting signals stimulated by bioactive substance into cells.
In a narrow sense, bio-signal transmitter means a substance which receives a signal from the receptor of a bioactive substance and regulates the expression of the gene in the nucleus
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Gene Therapy 
