 Peptide, a method for its preparation and a pharmaceutical composition containing the peptide |
| OF THE PREFERRED EMBODIMENT The following standard abbreviations for the amino acid residues are ... |
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| Mimotopes and anti-mimotopes of human platelet glycoprotein Ib/IX |
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| Adjuvant and vaccine compositions containing monophosphoryl lipid A |
| What is claimed: 1. A lyophilized composition comprising 0.2% up to 5% by weight 3-O-desacyl-4'-... |
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| Methods for preparation of vaccines against cancer |
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| Human genes and gene expression products V |
| OF THE INVENTION The invention relates to polynucleotides comprising the disclosed nucleotide ... |
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| Haptotactic peprides |
| The invention claimed is: 1. An isolated haptotactic peptide of 20 amino acids consisting of the ... |
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| Hepatitis C virus inhibitors |
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| Expression of recombinant glyoprotein B from herpes simplex virus |
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| In vitro packaging of adeno-associated virus DNA |
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Plant transcription regulators from circovirus
| Details |
Inventors: Boevink, Petra Christina; Surin, Brian Peter; Keese, Paul Konrad; Chu, Paul Wing Gay; Waterhouse, Peter Michael; Khan, Rafiqul Islam; Larkin, Philip John; Taylor, William Clark; Marshall, Jerry Stuart;
Assignee: Commonwealth Scientific and Industrial Research Organization (Campbell, AU)
Primary Examiner: McElwain; Elizabeth F.
Assistant Examiner: Mehta; Ashwin D.
Attorney, Agent or Firm: Scully, Scott, Murphy & Presser
The present invention is directed to transcription regulators and transcription regulator-like sequences of nanovirus origin. As used in the specification, the nanovirus group is consdiered to include subterranean clover stunt virus (SCSV), cocnut foliar decay virus (CFDV), banana bunchy top virus (BBTV), milk vetch dwarf cirus (MDV), and faba bean necrotic yellow virus (FBNYV). The transcription regulators and transcription regulator-like sequences of the instant invention are useful in genetic engineering of plants and in particular leguminous plants such as to facilitate or control expression of foreign genes. The transcription regulators and transcription regulator-like sequences of the present invention are also useful in facilitating different levels of expression in different plant tissue types. |
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DETAILED DESCRIPTION OF THE INVENTION The present invention is further described by reference to the following non-limiting figures and/or examples.
Reference herein to a promoter region from SCSV is abbreviated to "S" for SCSV, the genome segment number (e.
g.
1, 3, 4, 5 and 7) and "nc" for non-coding region.
For example, the promoter from SCSV genome segment 1 is defined as "S1nc".
Terminator sequences for particular SCSV genome segments are indicated for example, as follows: "SC1Tr" or "SC5Tr" for the terminator sequences for segments 1 and 5, respectively.
Genetic constructs comprising an SCSV promoter, a reporter gene such as GUS and a terminator sequence such as from SCSV is abbreviated to "SCSV:GUS:SCTr" or "SCSV:GUS:SCSVTr".
Specific promoters and termination sequences are defined as above, for example S4nc:GUS:SC1Tr or S4nc:GUS:Me3", "S4nc:GUS:Me3".
In the latter construct the terminator sequence from the MeA gene of Flaveria bidentis is used, referred to herein as "Me3".
In the Figures: FIG.
1 is a schematic representation showing the structures and transcription units found in a representative DNA component of a typical geminivirus and SCSV, both of which contain a ssDNA genome.
FIG.
2 is a schematic representation showing the seven DNA segments found in the genome of SCSV in a linear form, indicating the positions of the stem-loop structure, the common region, the open reading frame (ORF), the TATA box and the termination and polyadenylation signals on each DNA.
FIG.
3 is a schematic representation showing the construction of the seven SCSV DNA non-coding region: .
beta.
-glucuronidase (GUS) fusion expression vectors for transformation into tobacco plants.
The amplified PCR fragments were separately cloned in front of the GUS gene in pHW9 at the BamnHI (B) and NcoI (N) sites as indicated.
The resultant recombinant pHW9 vectors were cut at the EcoRI site and cloned into the EcoRI site of the recipient PGA470 binary vector.
FIG.
4 is a schematic representation showing the construction of the segments 5 and 7 promoter:GUS fusion expression vectors and their deletion derivatives for protoplast studies
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Gene Therapy 
