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Plant virus vector, plasmid, process for expression of foreign gene and process for obtaining foreign gene product
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Inventors: Hamamoto, Hiroshi; Sugiyama, Yoshinori; Nakagawa, Noriaki; Hashida, Eiji; Tsuchimoto, Suguru; Nakanishi, Noriyuki; Matsunaga, Yuji; Okada, Yoshimi;
Assignee: Kanebo Limited (Tokyo, JP)
Primary Examiner: Fox; David T.
Assistant Examiner:
Attorney, Agent or Firm: Sughrue, Mion, Zinn, Macpeak & Seas
The present invention relates to a plant virus vector comprising a foreign gene linked downstream of a coat protein gene of tobacco mosaic virus via a nucleotide sequence which cause the readthrough, and a plasmid which is transcribed to provide the vector, as well as a process for expression of a foreign gene in a plant by inoculating the plant with the vector. In addition, the present invention relates to a process for efficiently recovering a foreign gene product produced in a plant as virions. |
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DETAILED DESCRIPTION The purpose of the present invention is to provide plant virus vectors having the ability to systemically infect a whole plant; plasmids, which are transcribed to provide said plant virus vectors; and a process for expressing a foreign gene throughout a whole plant by inoculating said plant virus vector into a plant.
In addition, a process for simply and efficiently obtaining a foreign gene product produced in a plant is provided.
To accomplish the above-mentioned purposes, the present inventors use plant virus vector wherein a foreign gene is linked downstream of a coat protein gene of a plant virus via a nucleotide sequence that causes readthrough (Skuzeski et al, J.
Mol.
Biol.
, 218:365-373 (1991)); a plasmid that is transcribed to provide said plant virus vector; and infection throughout a whole plant (systemic infectivity) by inoculating said plant virus vector into a plant so as to express the foreign gene throughout the whole plant.
It was also found that introduction of a useful property into a whole plant and the production of a useful protein in a whole plant are possible, and thus, the present invention has been achieved.
In addition the present inventors found that when a foreign gene product is recovered from a plant, the foreign gene product can be easily and efficiently recovered as viral particles.
Prior to explaining the constituent features of the present invention, the terms used in the specification are explained as follows: Plant virus vector: a replicable recombinant product obtainable by introducing a foreign gene into a DNA or RNA sequence in a plant virus.
Readthrough: In translation of an RNA to a protein, a phenomenon wherein the translation does not stop at a stop codon, and sometimes continues to the next stop codon.
This readthrough phenomenon is caused by a nucleotide sequence near the stop codon.
Plant: in addition to an individual plant (a plant consisting of leaves, stem and roots); plant cells, callus, protoplast, as well as adventitious buds, adventitious roots and somatic embryos, derived from callus, are included, which are used as hosts for a plant virus
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Gene Therapy 
