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Probe for electrofusion, electroporation, or like procedure
| Details |
Inventors: Hilliard, Jeffrey L.; Moloney, Maurice M.;
Assignee: Hilliard; Jeffrey L. (Davis, CA)
Primary Examiner: Jones; Larry
Assistant Examiner:
Attorney, Agent or Firm: Townsend and Townsend
A probe and method for treating samples in a well tray. The probe is specially configured to engage the well, and when so engaged, to provide an electrode structure within the well. The probe comprises an outer electrode in the form of a metal ring having a diameter just slightly less than the inner diameter of the well, a center electrode in the form of a metal rod, and a non-conductive (typically clear plastic) cover that extends over the well and holds the electrodes coaxial with respect to each other and with respect to the well. A metal pin is rigidly connected to the ring and extends parallel to the ring axis. The rod and pin pass through the cover so that their upper ends are accessible for electrical connections. Electrical connections are made to the portions of the rod and the pin located above the cover. As a safety measure, the electrical connections are preferably hidden under a cap that threadingly engages the top portion of the rod and overlies the cover. |
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DETAILED DESCRIPTION The present invention provides a structure and method for treating samples in a well tray.
Briefly, the invention contemplates the provision of a probe that is specially configured to engage the well, and when so engaged, to provide an electrode structure within the well.
The probe comprises an outer electrode in the form of a metal ring having a diameter just slightly less than the inner diameter of the well, a center electrode in the form of a metal rod, and a non-conductive (typically clear plastic) cover that extends over the well and holds the electrodes coaxial with respect to each other and with respect to the well.
A metal pin is rigidly connected to the ring and extends parallel to the ring axis.
The rod and pin pass through the cover so that their upper ends are accessible for electrical connections.
A second pin, also connected to the ring, may be provided to engage the cover in order to help maintain the ring coaxial with the rod.
The cover is preferably in the form of a flat portion extending beyond the edges of the well and one or more projecting portions configured to engage the inner surface of the well.
Thus, when the cover sits on top of the well, the projecting portions extend into the well and provide transverse registration to the well.
In a preferred embodiment, the projecting portion is a single circular projection of a diameter slightly less than the well diameter.
Electrical connections are made to the portions of the rod and the pin located above the cover.
As a safety measure, the electrical connections are preferably hidden under a cap that threadably engages the top portion of the rod and overlies the cover.
Thus connection to the rod and pin can only be made from the side, with no metal portions exposed.
In a second embodiment, a single cover, large enough to span a plurality of wells, carries a corresponding plurality of electrode pairs spaced according to the well spacing.
This allows multiple samples to be treated at one time.
A bus bar arrangement connects all the rods to each other and all the pins to each other, so that only one set of electrical connections needs to be made
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