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Gene Therapy

Solely-pollen-specific-promoter

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Solely pollen-specific promoter

Details

Inventors: Shin, Jeong Sheop; Ryu, Beung Tae; Bahn, Sung Chul; Kim, Hae Jin;
Assignee: Korea University Industry& Academy Cooperation Foundation (Seoul, KR)
Primary Examiner: Mehta; Ashwin D.
Assistant Examiner: Worley; Cathy Kingdon
Attorney, Agent or Firm: St. Onge Steward Johnston & Reens LLC

An isolated solely pollen-specific promoter derived from Arabidopsis comprise a nucleotide sequence of Arabidopsis PLA.sub.2-.delta. gene. The novel promoter has specific roles in particular organs and at particular developmental stages, such as in mature flowers, and can be used in the production of hybrid seeds using male sterility techniques.

DETAILED DESCRIPTION What is claimed is: 1.
An isolated solely pollen-specific promoter derived from Arabidopsis comprising a complete nucleotide sequence of SEQ ID NO: 1 derived from the Arabidopsis AtsPLA.
sub.
2-.
delta.
gene and having a size of about 550 base pair.
2.
A recombinant vector comprising an isolated solely pollen-specific promoter derived from Arabidopsis comprising a complete nucleotide sequence of SEQ ID NO: 1 derived from the Arabidopsis AtsPLA.
sub.
2-.
delta.
gene and having a size of about 550 base pair; and a foreign gene fused with the said promoter and regulated by the promoter.
3.
The recombinant vector according to claim 2, wherein the foreign gene is a gene degrading male fertility.
4.
A plant cell transformed by the recombinant vector according to claim 2.
5.
A plant cell transformed by the recombinant vector according to claim 3.
6.
The isolated pollen-specific promoter of claim 1, wherein the promoter is amplified by a primer comprising a complete nucleotide sequence of SEQ ID NO: 4 and SEQ ID NO: 5.

Description:

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