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Gene Therapy

Superfusion-apparatus

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Superfusion apparatus

Details

Inventors: Bertoncini, Joseph; Kroener, Calvin; Cobb, Wayne; Evans, Robert;
Assignee: Biomedical Research And Development Laboratories, Inc. (Gaithersburg, MD)
Primary Examiner: Warden; Robert J.
Assistant Examiner: Trembley; T. A.
Attorney, Agent or Firm: Breneman & Georges

A superfusion apparatus for the controlled reaction of a sample within a test chamber through controlled exposure to a series of reagents in a set test sequence is provided for electrical or chemical stimulation to determine if a receptor binding compound is an agonist or antagonist. The superfusion apparatus provides a mass production but meticulously controlled sample environment for controlling temperature, quantity and arrangement of reagents and wash fluids to provide a high reproducability of results which may be compuer assisted. The device has a fully automated mode or a manually operated mode. The fully automated mode allows for the storage and execution of a number of different tests to be performed on the samples due to the provision of desired sequences of test parameters.

DETAILED DESCRIPTION It is an object of the present invention to provide a superfusion apparatus for use in receptor binding procedures providing a higher degree of control features amenable to both chemical and electrical procedures for determining the binding of a radioactive ligand at a receptor site with a high degree of reproduceability to assess the quality of the resultant binding.
It is a further object of the present invention to provide a superfusion device with simultaneous multiple binding capability which can discriminate between acceptable and unacceptable bindings through the utilization of chemical and/or electrical stimulation.
It is another object of the present invention to provide a superfusion device for use in receptor binding procedures which can evaluate the quality of the bond produced utilizing chemical and/or electrical stimulation.
It is still another object of the present invention to provide a superfusion device which can simultaneously perfuse a multiplicity of tissues with a selective variety of a number of different reactants.
It is yet a further object of the present invention to provide a superfusion device which can simultaneously perfuse a multiplicity of tissues with a variety of reagents in a preselected specified sequence.
It is another object of the present invention to provide a highly automated multichannel, multicell superfusion device with multiple simultaneous perfusion capabilities which provides for acquisition of a multitude of separate effluents corresponding to each perfusion site with minute quantities of reagents precisely controlled utilizing automated procedures.
These and other objects of the present invention are accomplished by providing a superfusion device of multiple channel design which allows for simultaneous perfusion of a number of tissues with any one or a multiplicity of a selected number of reagents.
The device allows for an automated and computer controlled superfusion apparatus for the controlled and selected sequencing of the introduction of reactants in the perfusion operation

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