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Home Graphic Cards Method-of-photometric-in-vitro-determination-of-the-content-of-oxygen-in-a-blood-sample

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 Method of photometric in vitro determination of the content of oxygen in a blood sample

Details
Inventors: Lundsgaard, Finn C.; Jensen, Niels-Henrik; Andersen, Willy;
Assignee: Radiometer A/S (Bronshoj, DK)
Primary Examiner: Sykes; Angela D.
Assistant Examiner:
Attorney, Agent or Firm: Bryan Cave LLP

In a method of photometric in vitro determination of the content of oxygen in a blood sample, a blood sample is transferred directly from an in vivo locality to an at least partially transparent sample container of a sampling device. The sample container has a measuring chamber containing a luminophor, the luminescence of which is quenched in the presence of oxygen. The luminophor is excited by irradiation with radiation from a radiation source. The luminescence emitted by the excited luminophor is detected by a radiation detector and the oxygen content determined on the basis of the radiation detected by the radiation detector. A sampling device and a system for photometric in vitro determination of oxygen in a blood sample are also described.

DETAILED DESCRIPTION The object of the invention is to provide an in vitro method for determination of the content of oxygen in a blood sample, the method being more appropriate for the user in that there is obtained both a more simple and less risky sample handling and a more simple maintenance of the oxygen analyzer utilized in practising the method.
This is achieved by the method according to the invention, comprising transferring a blood sample from an in vivo locality to the sample container of a sampling device, the sample container having a measuring chamber with an at least locally transparent wall part and containing a luminophor; breaking the connection between the sampling device and the in vivo locality after filling the sample container with the blood sample; bringing the measuring chamber into optical communication with an optical system comprising a radiation source and a radiation detector; exciting the luminophor within the measuring chamber by irradiation with radiation from the radiation source; and determining the luminescence characteristic of the luminescence emitted from the luminophor based on the luminescence detected at the radiation detector.



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