 High efficiency fuser roll assembly for xerographic material |
| The present invention relates to apparatus for making multiple copies of printed or other matter by ... |
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| Backup roll for heated fuser system |
| OF THE PREFERRED EMBODIMENT As shown in FIG. 1, the fuser system includes a fuser roll couple ... |
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| Roll-fusing apparatus |
| It is an object of the present invention to equalize the characteristic of contact pressure exerted ... |
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| Portable heat circulation means |
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| Interior trim foam product and method of fabrication thereof |
| OF THE INVENTION The article of the present invention and its method of manufacture will be ... |
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| Apparatus and method for removing fumes from the space above a cooking appliance in a restaurant |
| According to the present apparatus and method, substantially uniform supply and exhaust velocities ... |
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| Food receptacle for microwave ovens |
| What is claimed is: 1. A microwave oven for cooking food comprising: an oven for heating said food, ... |
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| Interruptable voltage-controlled oscillator and phase-locked loop using same |
| OF THE PREFERRED EMBODIMENTS Reference is now made in detail to the present preferred embodiments ... |
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| Varying frequency oscillator for the reduction of radiated emissions of electronic equipment |
| These and other objects of the invention are accomplished by providing a circuit which varies the ... |
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| High frequency ECL voltage controlled ring oscillator |
| OF THE INVENTION As shown in FIG. 4, the present invention provides an ECL gate configuration 100 ... |
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Reagent and process for the determination of the activity of the enzyme gamma-glutamyl transferase
| Details |
Inventors: Deneke, Ulfert; Nagel, Rolf; Rittersdorf, Walter;
Assignee: Boehringer Mannheim GmbH (Mannheim, DE)
Primary Examiner:
Assistant Examiner:
Attorney, Agent or Firm:
The present invention provides a process for the kinetic determination of .gamma.-glutamyl transferase activity by the reaction of a .gamma.-glutamylarylamide with the formation of an arylamine at a pH value of from 6 to 10, wherein the arylamine is simultaneously reacted with an aromatic amine or phenol appropriate as a coupling component and an appropriate oxidation agent to give a colored compound with an absorption maximum above 565 nm, the rate of formation of which is monitored photometrically and is used as a measure for the .gamma.-glutamyl transferase activity. The present invention also provides a reagent for the kinetic determination of .gamma.-GT activity, wherein it contains the following reagents: .gamma.-GT-arylamide:1-100 mmol/l. .gamma.-glutamyl acceptor (glycylglycine):10-500 mmol/l. coupling component:1-500 mmol/l. oxidation agent:0.1-500 mmol/l. buffer:10-1000 mmol/l. |
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DETAILED DESCRIPTION What is claimed is: 1.
Process for the kinetic determination of gamma-glutamyl transferase (gamma-GT) activity by the reaction of a gamma-glutamylarylamide substrate (gamma-GT substrate) with a gamma-glutamyl acceptor and gamma-GT comprising simultaneously combining the gamma-GT substrate with an oxidation agent, gamma-GT, an aromatic amine coupling component or a phenol coupling component, and a gamma-glutamyl acceptor said combination taking place at a pH of from about 6 to 10, said gamma-GT substrate forming an arylamine which reacts with said coupling component to give a colored compound with an absorption maximum above 565 nm, and photometrically monitoring the rate of formation of said colored compound as a measure of activity of the gamma-GT.
2.
The process of claim 1 wherein the oxidation agent is a ferricyanide or a peroxide/peroxidase.
3.
The process of claim 1 wherein the gamma-GT substrate is a compound of the formula ##STR3## wherein X is an --NR.
sub.
2 R.
sub.
3 or --O--R.
sub.
2 group; R.
sub.
1 is hydrogen; alkoxy, alkyl or aryl containing up to 8 carbon atoms; chlorine, bromine or iodine; or --NR.
sub.
2 R.
sub.
3, --SO.
sub.
3 R.
sub.
2 or --COOR.
sub.
2 ; and R.
sub.
2 and R.
sub.
3, which are the same or different, are hydrogen; or substituted or unsubstituted alkyl or aryl containing up to 8 carbon atoms with substituents selected from the group consisting of OH, alkoxy, COOH and SO.
sub.
3 H.
4.
A process as claimed in claim 1 wherein the coupling component is a compound of the formula ##STR4## wherein X is --NR.
sub.
2 R.
sub.
3 or --O--R.
sub.
3 ; R.
sub.
1 is hydrogen, or chlorine when R.
sub.
4 and/or R.
sub.
6 are also chlorine; R.
sub.
2 and R.
sub.
3 are individually selected from the group consisting of hydrogen; or substituted or unsubstituted alkyl, aralkyl, or aryl containing up to 8 carbon atoms, with the substituent selected from the group consisting of hydroxyl, alkoxy containing up to 5 carbon atoms, --COOH or --SO.
sub.
3 H; R.
sub.
4 and R.
sub.
6 are individually selected from the group consisting of hydrogen, alkyl containing up to 5 carbon atoms, chlorine, bromine, iodine, --COOR
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Heat Accumulators 
