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Photographing apparatus balancing brightness for microscopes
| Details |
Inventors: Takabayashi, Yutaka; Utsunomiya, Hiromi; Nagano, Takashi; Kojima, Jitsunari;
Assignee: Olympus Optical Co., Ltd. (Tokyo, JP)
Primary Examiner: Nelms; David C.
Assistant Examiner: Messinger; Michael
Attorney, Agent or Firm: Cushman, Darby & Cushman
A photographing apparatus for microscopes is constructed so that a simultaneous observation is made by overlapping an image of a sample formed by transmitted illumination with that formed by reflected fluorescence, in which signals for designating the simultaneous observation at once cause a second shutter for the transmitted illumination to be closed and a first shutter for the reflected fluorescence to be opened, the brightness of the sample image formed by the reflected fluorescence is measured by a light-detecting element disposed in an optical path for observation, and after a light-adjusting member for the transmitted illumination is controlled so that, based on data outputted from a memory device corresponding to a measured value, the brightness of the sample image by the transmitted illumination in the simultaneous observation is balanced with that of the sample image by the reflected fluorescence, the second shutter is opened. Thus, this photographing apparatus has advantages that whenever the microscopy is switched, an operator can dispense with the need for a fine adjustment of the amount of transmitted illumination light, with the resultant ease of operation, the balance adjustment of brightness in switching is made in a short time, and the bleaching of fluorescence of the sample can be suppressed. |
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DETAILED DESCRIPTION It is, therefore, an object of the present invention to provide a photographing apparatus for microscopes which enables the simultaneous observation on transmission and reflection to be made so that the adjustment of the balance of brightness in the simultaneous observation is made in a short time and with simple operation, and bleaching of fluorescence of the sample can be suppressed.
To achieve this object, and in accordance with one aspect of the present invention, the photographing apparatus for microscopes is constructed so that the simultaneous observation can be carried out by overlapping the sample image formed by transmitted illumination light with that by reflected fluorescence, in which signals for designating the simultaneous observation at once cause a shutter disposed in the optical path for transmitted illumination to be closed and another shutter disposed in an optical path for reflected illumination to be opened, the brightness of the sample image formed by the reflected fluorescence is measured by a light-detecting element disposed in an observation optical system, and after a light-adjusting member disposed in the optical path for transmitted illumination is controlled so that, based on data read out from a memory section corresponding to the measured value, the brightness of the sample image by the transmitted illumination light in the simultaneous observation is balanced with that of the sample image by the reflected fluorescence, the shutter disposed in the optical path for transmitted illumination is opened.
Thus, for the simultaneous observation, the reflected fluorescence observation is first made in which the brightness of the sample image by the reflected fluorescence is measured by the light-detecting element, relational operations on the measured value and the data from the memory section are performed, the light-adjusting member is controlled so that the brightness of the sample image by the transmitted illumination light is balanced with that by the reflected fluorescence, the brightness of the sample image in the optical path for transmitted illumination is adjusted in a closed state of the shutter, and when the shutter is opened, the sample images by the reflected fluorescence and the transmitted illumination light can be observed at once and with mutually balanced brightness
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Image Analysis 
