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Home Lighting Process-and-apparatus-for-the-detection-of-toxicity-in-surface-waters-as-well-as-drinking-water-and-industrial-water

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 Process and apparatus for the detection of toxicity in surface waters as well as drinking water and industrial water

Details
Inventors: Krause, Hans; Maske, Helmut;
Assignee:
Primary Examiner: Kepplinger; Esther L.
Assistant Examiner: Scheiner; Toni R.
Attorney, Agent or Firm: Weingarten, Schurgin, Gagnebin & Hayes

This invention provides a process and an apparatus for the detection of toxicity in surface waters, by which the fluorescence of a water sample is measured in that the correlation, especially the ratio, between the prompt fluorescence and retarded fluorescence of the water sample is determined, which contains a toxicity-sensitive bioorganism. The apparatus comprises a combination of a first fluorescence measuring means for measuring prompt fluorescence and a second fluorescence measuring means for measuring retarded fluorescence as well as a signal correlation means for correlating the output signals of two fluorescence measuring means with each other to form an output quantity indicating the toxicity of the water sample.

DETAILED DESCRIPTION We claim: 1.
A process for the detection of toxicity in water, comprising the steps of: stimulating a water sample containing a toxicity-sensitive bioorganism by means of light having at least one predetermined wavelength; measuring the intensity of prompt fluorescence in the light-stimulated water sample containing said bioorganism; providing an output value corresponding to the measured intensity of prompt fluorescence; measuring the intensity of delayed fluorescence of the light-stimulated water containing said bioorganism synchronously with said measuring of prompt fluorescence intensity; providing an output value corresponding to the measured intensity of delayed fluorescence; correlating said output value corresponding to the measured intensity of prompt fluorescence with said value corresponding to the measured intensity of delayed fluorescence to obtain a correlated sample value; and providing an indication of said correlated sample value compared with a correlated sample value obtained from a non-contaminated sample, as a measure of the toxicity level of said light-stimulated water.
2.
The process of claim 1 wherein said correlating step includes measuring within a predetermined time window starting from the end of influence of the stimulating light for the retarded fluorescence on the sample, this time window defined between 0.
1 to 500 seconds after the end of said stimulating light for the retarded fluorescence.
3.
The process of claim 1 wherein the toxicity-sensitive bioorganism is selected from the group consisting of planktons, phytoplanktons, bacteria and dinoflagellates.
4.
The process of claim 1 wherein the light-stimulating step includes using light having at least two predetermined wavelengths.
5.
The process of claim 1 wherein said light-stimulating step includes using light having a wavelength of 440 nm for stimulating prompt and retarded fluorescences.
6.
The process of claim 1 wherein said light-stimulating step includes using light having a wavelength range between 660 nm to 685 nm for stimulating prompt and retarded fluorescences



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