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Activated polymer solid bodies and processes for the production thereof
| Details |
Inventors: Schossler, Werner; Boeden, Hans-Friedrich; Holtzhauer, Martin; Loth, Fritz; Hiepe, Falk; Bertram, Dieter; Mielke, Frank; Muller, Reinhard; Konjuchowa, Dagmar;
Assignee: Veb Leipziger Arzeimittelwerk (Leipzig, DD)
Primary Examiner: Marquis; Melvyn I.
Assistant Examiner:
Attorney, Agent or Firm: Striker; Michael J.
A chemically modified and activated, hydroxyl-group-containing, natural or synthetic, polymeric solid body surface is disclosed, as well as a process for the activation of such solid body surfaces by means of organo-silanes and, if necessary, homo- or heterobifunctional reagent. The surfaces are used for the stable, simple and economical binding of proteins, nucleic acids, low-molecular ligands, cells, microorganisms and other biological materials, in biology, biotechnology and medicine. |
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DETAILED DESCRIPTION What is claimed as new and desired to be protected by Letters Patent is set forth in the appended claims: 1.
Process for the activation of hydroxyl group-containing polymeric solid body carrier material surfaces, comprising reacting said hydroxyl groups to form chemical bonding with an organo-silane of the formula (I) (XR'.
sub.
n').
sub.
n SiR.
sub.
4-n (I) in which X carbonyl, carboxy, epoxy, isocyano, isothiocyano, nitroso, sulfhydryl or halocarbonyl, R' is alkyl, alkylphenyl or phenyl, R is alkoxy, phenoxy or halogen, n' is an integer from 0 to 20 and n is an integer from 1 to 3, or with two or more organo-silanes of formula (I), said two or more organo-silanes being provided together in mixture, or successively, said organo-silane being provided in a fluid phase.
2.
The process according to claim 1, wherein said reacting hydroxyl groups with organo-silane is effected in solution selected from the group consisting of organic solvent, organic solvent mixtures, aqueous solution and aqueous/organic solvent mixtures.
3.
The process according to claim 1, wherein said reacting hydroxyl groups to form chemical bonding with organo-silane is effected in gaseous phase by means of aerosol spraying said organo-silane onto said carrier matrix surfaces.
4.
The process according to claim 1, wherein said reacting hydroxyl groups with organo-silane is effected at a pressure below atmospheric pressure.
5.
The process according to claim 1, wherein said polymeric solid body comprises a shaped body.
6.
The process according to claim 5, wherein said shaped body comprises a spherical, fiber-like or cornered shape.
7.
The process according to claim 5, wherein said shaped body comprises a ball shape.
8.
The process according to claim 5, wherein a plurality of said shaped body are grouped together by means of packing, interweaving or binding agent into column packing or laminar material.
9.
The process according to claim 5, wherein said shaped body is provided in the form of a thin layer as a film or lacquer on other carriers
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Liquid Purification 
