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Details
Inventors: Schels, Hans; Menzler, Horst; Fischer, Ulrike;
Assignee: Roche Diagnostics GmbH (DE)
Primary Examiner: Redding; David A.
Assistant Examiner:
Attorney, Agent or Firm: Waite; Kenneth J. Roche Diagnostics Corporation

A bioreaction module for biochemical reactions, in particular for cell-free polypeptide biosynthesis, having a housing (1) including a system chamber (12) and a supply chamber (10), wherein the system chamber (12) contains a producing system during the biochemical reaction and the supply chamber (10) contains a supply liquid during the biochemical reaction, and the system chamber (12) and the supply chamber (10) are separated by a semi-permeable membrane (7). The housing (1) comprises a system chamber element (5) and at least one supply chamber element (3) between which the semi-permeable membrane (7) is mounted in such a manner that the system chamber (12) is defined by the system chamber element (5) and the semi-permeable membrane (7) and the supply chamber (10) is defined by the supply chamber element (3) and the semi-permeable membrane (7).

DETAILED DESCRIPTION We claim: 1.
A bioreaction module for biochemical reactions, said module comprising a system chamber, one or more supply chambers, and one or more semi-permeable membranes that allow bidirectional exchange of products and liquids between the system chamber and the one or more supply chambers; wherein the system chamber is defined by a system chamber element and one or more of said semi-permeable membranes and said system chamber contains a producing system during the biochemical reaction; wherein each supply chamber is defined by a supply chamber element and one of said semi-permeable membranes which separate the supply chamber from the system chamber and said supply chamber contains a supply liquid during the biochemical reaction; said module further comprising sealing means engaging a peripheral marginal section of said one or more semi-permeable membranes, said sealing means comprising a peripheral groove in either the supply chamber element or the system chamber element and a protruding rib in the other chamber element for penetrating into the groove to push the membrane at its marginal section into the groove.
2.
A module according to claim 1 wherein one of the chamber elements has a fill opening in a wall opposite to the semi-permeable membrane for filling the chamber defined by said chamber element.
3.
A module according to claim 2 wherein both the system chamber element and the one or more supply chamber elements have a fill opening for filling the chamber defined by the respective chamber element, that fill opening being located in a wall opposite to the membrane.
4.
A module according to claim 3 wherein the walls of the system chamber element and of the supply chamber element containing the fill openings are each configured as seating surfaces for the module and are oriented such that when the module seats on one of these seating surfaces, the other respective chamber can be filled.
5.
A module according to claim 2 wherein the wall opposite from the semi-permeable membrane has both a fill opening and an additional vent opening



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