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Method and apparatus for separating mononuclear cells from blood
| Details |
Inventors: Smith, Ward C.;
Assignee: Becton Dickinson and Company (Franklin Lakes, NJ)
Primary Examiner: Jones; W. Gary
Assistant Examiner:
Attorney, Agent or Firm: Hoffmann & Baron
A blood separation device includes a collection tube containing a first layer of a thixotropic gel-like substance and a second layer of a newtonian gel-like substance which is positioned below the first layer. The first layer of the thixotropic substance is positioned between the second layer of the newtonian substance and a blood sample received by the tube, prior to centrifuging the tube, and acts as a barrier between the second layer and the blood sample to prevent intermixing of the second layer and the blood sample. The thixotropic gel-like substance of the first layer has a specific gravity which is greater than the newtonian gel-like substance of the second layer whereupon centrifugation of the tube, the first layer moves to a position below the second layer. The second layer of the newtonian gel-like substance acts as a density separation medium, the newtonian gel-like substance being formed with a specific gravity such that the second layer is adapted to assume a position in the collection tube, upon centrifugation of the tube, between the mononuclear cells and the heavier components of the blood sample placed in the tube. |
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DETAILED DESCRIPTION It is an object of the present invention to provide a blood separation device which is shippable.
It is another object of the present invention to provide a method for separating the mononuclear cells from the other components of a blood sample.
It is a further object of the present invention to provide a blood separation device and method for using the same which overcomes the inherent disadvantages of known separation devices, such as that described previously.
In accordance with one form of the present invention, a blood separation device, and in particular one which is adapted for separating mononuclear cells from whole or diluted blood, includes a collection tube having a bottom end which is closed and an opposite top end which is open for receiving a blood sample.
The collection tube is adapted to be centrifuged.
The device further includes a first layer of a thixotropic gel-like substance contained in the collection tube, and a second layer of a newtonian gel-like substance, also contained in the collection tube and positioned below the first layer.
The collection tube is formed to be of a sufficient size to define a free space above the first layer of the thixotropic substance to receive the blood sample.
The first layer of the thixotropic substance is positioned between the second layer of the newtonian substance and a blood sample received by the tube, prior to centrifuging the tube, and acts as a barrier between the second layer of the newtonian substance and the blood sample to prevent the intermixing or intermingling of the second layer and the blood sample.
Accordingly, the thixotropic gel will not run and will maintain the separation of the blood sample and the newtonian gel even when the tube is placed on its side, as during shipment from physician to laboratory.
The thixotropic gel-like substance of the first layer has a specific gravity which is greater than that of the newtonian gel-like substance of the second layer.
Upon centrifugation of the tube, the first layer moves to a position below the second layer of the newtonian substance
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