 Blood centrifuge having integral heating to control cellular component temperature |
| Accordingly, one object of this invention is to provide a method and apparatus for the separation ... |
|
| Method and apparatus for controlling the washing step in a blood centrifugation cell |
| The invention provides a system for controlling the washing step in which the washing step can be ... |
|
| Folding machine with stacking arm |
| The present invention provides for a folding machine comprising a housing having a fold plate ... |
|
| In-line separator for crude oil |
| OF THE PREFERRED EMBODIMENT Referring now to FIG. 1, an embodiment of the device of the present ... |
|
| Magnetic fuel ion modifier |
| I claim: 1. A liquid fuel ion modifier for use between a fuel supply and a fuel consuming device ... |
|
| Magnetic fluid conditioner |
| One embodiment of our invention shown in FIGS. 1-5 includes a conduit 10 having a middle section ... |
|
| Method and a device for separating a continuous fluid phase from a dispersed phase |
| The present invention concerns a method for separating a continuous phase from at least one ... |
|
| Water clarification system |
| The invention is defined by the appended claims with specific embodiments shown in the attached ... |
|
| Compact, efficient, monitorable immiscible fluid separator |
| An object of the present invention is to provide an improved, immiscible fluid separator that is ... |
|
|
Method of isolating biomolecules by ion exchange
| Details |
Inventors: Lihme, Allan Otto Fog; Aagesen, Margit Irene; Gammelg.ang.rd-Larsen, Claus; Elleg.ang.rd, Katrine Hvid;
Assignee: Kem-En-Tec A/S (Copenhagen O, DK); Md Foods AMBA (Viby J, DK)
Primary Examiner: Robinson; Douglas W.
Assistant Examiner: Mohamed; Abdel A.
Attorney, Agent or Firm: Finnegan, Henderson, Farabow, Garrett & Dunner, L.L.P.
A method of isolating a biomolecule from a medium containing biomolecules by ion exchange wherein the ion exchange material consists of a material having ion exchanging groups which can be transformed from a charged form to an uncharged form; the eluant comprises a charge neutralizing acid or base transforming the ion exchanging groups from the charged form to the uncharged form; and the charge neutralizing acid or base has a concentration in the eluant which is twice, preferably equal to or less than the concentration of the ion exchanging groups of the ion exchange material; the ion exchange material being in a packed, hydrated state. Preferably the method is for the isolation of phosphopeptides from a medium containing casein hydrolysates and the use of such isolated biomolecules is for the production of a food, a feed, a health care product, a cosmetic, or a pharmaceutical. |
|
DETAILED DESCRIPTION Ion Exchange Using A Weak Ion Exchanger Referring to Figs.
1A-C sketches are shown of ion exchange columns containing a weak ion exchanger (anion exchanger) to which biomolecules have been loaded.
In FIG.
1A biomolecules have been loaded at the top, A, on to the column at a pH about 4, whereafter unbound biomolecules are washed out (in the direction of the arrow, F) at this pH through the bottom, B.
The pH of the mobile phase is about 4 as indicated by the curve 21, and the concentration of unbound biomolecules is zero as indicated by the curve 22.
In FIG.
1B the bound biomolecules are eluted (in the direction of the arrow, F) by means of an eluant containing a charge neutralizing'acid or base (here a base) having a concentration which is much larger than the concentration of the ion exchanging groups of the ion exchange material.
As the eluant moves down, the ion exchanging groups are immediately neutralized by reaction with the base and the bound biomolecules become unbound biomolecules as indicated by the curve 22.
Due to the excess of the charge neutralizing base, the pH of the mobile phase rises very rapidly to an extreme pH value as indicated by the curve 21.
Under these conditions a major fraction of the unbound biomolecules is exposed to an environment of extreme pH, e.
g.
the fraction of biomolecules under the elution peak indicated by the area 23 is exposed to a pH above 10.
A similar situation exists for ion exchange using a weak cation exchanger, wherein the eluant contains a charge neutralizing acid having a concentration which is much larger than the concentration of the ion exchanging group of the ion exchanger.
In this case the major fraction of the unbound biomolecules are exposed to low pH.
In either case the exposure of the biomolecule to extreme pH values may damage their biological and physico-chemical properties.
This problem can be avoided by elution by means of an eluant containing a charge neutralizing acid or base (here a base) having a concentration according to the invention which is twice, preferably equal to or less than the concentration of the ion exchanging groups of the ion exchange material
|
|
Liquid Purification 
