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Miniaturized flow thermocycler
| Details |
Inventors: Baier, Volker; Bodner, Ulrich; Dillner, Ulrich; Kohler, Johann Michael; Poser, Siegfried; Schimkat, Dieter;
Assignee: Biometra Biomedizinische Analytik GmbH (Goettingen, DE)
Primary Examiner: Redding; David A.
Assistant Examiner:
Attorney, Agent or Firm: Jordan and Hamburg
A miniaturized thermocycler is provided for carrying out thermally controlled biochemical or biological melecular processes, in particular polymerase chain reactions. The aim of the invention is to provide a miniaturized thermocycler which enables such reactions to be carried out more effectively, avoids the problem of parasitic heat absorbers and can be manufactured inexpensively in series. This aim is achieved by virtue of the fact that the sample holder (1) is designed as a series of meanders winding in a plane, the sample holder (1) has a groove in a wall and closed over by a cover, the groove passing alternately through comparable heating zones (2) and cooling zones (3), located at intervals along the groove, as it meanders. |
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DETAILED DESCRIPTION It is an object of the present invention provide a miniaturized thermocycler which, compared to the state of art, permits to more effective control of biochemical and biological molecular, respectively, processes and, in particular, the method for polymerase chain reaction, which obviates the problem of parasitic heat capacities and which is inexpensive in manufacture.
The invention is based on the idea to utilize structurizing technologies known from so-called microsystem techniques in order to provide reaction chambers which permit dynamic reaction treatment of even very low amounts of, partially, very expensive materials.
An embodiment of receptacle portions for receiving a sample material ensures that the sample portion volumes under treatment in respective heating and cooling zones are subjected to a homogeneous temperature which also increases the output of the substance to be amplified.
Furthermore, due to the omission of heating and cooling processes of wall materials by virtue of the arrangement, and due to a severe minimizing of the parasitic heat absorption and heat affects, the expenditures for control systems are considerably reduced, apart from a remarkable reduction of time for the entire cycling process.
Only as much heating and cooling power has to be fed in as carried in the reaction liquid stream.
Additionally, the embodiment of the thermocycler not only permits a continuous process but also a serial operation in which different substances are subsequently injected into the thermocycler without the danger of mixing with sample materials still in the arrangement which is simply obtained by inserting a small buffer gas volume.
The abovementioned advantages also ensure an automation of the method for polymerase chain reaction.
Furthermore, an easy combination with other methods is feasible such as the micro-gel-electrophoresis, the micro-capillary-chromatography and other micro separating and analyzing methods.
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MEMS 
