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Microbiological-assembly-comprising-resealable-closure-means

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Microbiological assembly comprising resealable closure means

Details

Inventors: Brown, James F.;
Assignee: Cytonix Corporation (Beltsville, MD)
Primary Examiner: Redding; David A.
Assistant Examiner:
Attorney, Agent or Firm: Kilyk & Bowersox, P.L.L.C.

A microbiological assembly is provided having a resealable seal between a support and a cover. The support has a surface which includes a patterned layer and defines at least one sample retention well. The cover has a surface which contacts a sealant and the sealant is disposed on at least one of the support and the cover. The sealant is adapted to releasably bond and resealably seal the cover to the support. The sealed assembly minimizes contamination and drying of liquid sample retained by the sample retention well. A method of examining a biological culture is also provided and includes the steps of retaining a biological sample within the sample retention well of the assembly, sealing the cover to the support with the sealant between the sealing surface and the support surface, physically separating the cover from the support surface, and then either resealing the cover or sealing a second cover to the support using the same sealant. Assemblies including magnetic, electrostatic and other resealable seals are also provided.

DETAILED DESCRIPTION The present invention provides a microbiological assembly which is covered with a resealable cover that reduces evaporation of costly reagents, minimizes assay contamination, and eliminates the need to store such slides in humid incubation chambers.
The present invention also provides a microbiological or chemical reaction assembly having a resealable closure means that is used for sealing a first cover and then sealing a second cover after the first cover is released from the closure means.
It is an object of the present invention to provide a multi-chambered device for growing cells and performing assays on a flat planar surface.
It is a further object of the present invention to provide a multi-chambered device for growing cells and conducting assays using very small volumes.
It is a further object of the present invention to provide a multi-chambered device for growing cells and performing assays which has a resealable cover of any of various dimensions.
It is a further object of the present invention to provide a multi-chambered device for growing cells and performing assays wherein the device may have a permanent cover of any of various dimensions.
It is a further object of the present invention to provide a multi-chambered device for growing cells and performing an assay wherein the device has a cover which attaches to a support by means of a pressure sensitive adhesive which is resistant to laboratory chemicals used for the cell growth and the assay.
It is a further object of the present invention to provide a multi-chambered device for growing cells and performing an assay wherein the device has a cover which attaches to a support by means of a pressure sensitive adhesive which is resistant to laboratory chemicals used in the cell growth and the assay, and, when in contact with a hydrophobic surface, forms a virtually leak-proof seal.
It is yet a further object of the present invention to provide a multi-chambered device for growing cells and conducting assays which is both inexpensive and disposable

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