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Acidic uricase and process for production thereof
| Details |
Inventors: Nakanishi, Toru; Shigemasa, Yoshimi;
Assignee: Kyowa Hakko Kogyo Co., Ltd. (Tokyo, JP)
Primary Examiner: Shapiro; Lionel M.
Assistant Examiner:
Attorney, Agent or Firm: Fitzpatrick, Cella, Harper & Scinto
Acidic uricase is produced by fermentation of a microorganism of the genus Streptomyces. The enzyme is useful for the quantitative determination of uric acid in a sample. |
|
DETAILED DESCRIPTION What is claimed is: 1.
Acidic uricase having an optimum pH in the range of 4.
7 to 5.
1, and characterized by catalyzing the oxidation of uric acid to allantoin.
2.
A process for producing acidic uricase which comprises culturing a microorganism belonging to the genus Streptomyces which is capable of producing acidic uricase having an optimum pH in the range of 4.
7 to 5.
1, and characterized by catalyzing the oxidation of uric acid to allantoin in a nutrient medium until enzymatic activity is detected in the culture liquor and thereafter recovering said acidic uricase therefrom.
3.
A process according to claim 2 wherein said microorganism belongs to the species Streptomyces gannmycicus.
4.
A process according to claim 3 wherein said microorganism has the identifying characteristics of Streptomyces gannmycicus ATCC 27434.
5.
A process according to claim 2 wherein said culturing is carried out at 25.
degree.
to 35.
degree.
C.
for 1 to 3 days at about neutral pH.
Description:
BACKGROUND OF THE INVENTION The present invention relates to uricase having an optimum pH in the acidic region (hereinafter referred to as acidic uricase), a process for production thereof, a method for the quantitative determination of uric acid in a sample by enzymatic reaction and a test composition suitable therefor.
Uricase is a known enzyme which catalyzes the hydrolysis of uric acid to allantoin, hydrogen peroxide and carbon dioxide.
The enzyme is, therefore, useful for the determination of uric acid in blood, urine, and the like.
Known uricases have an optimum pH in the neutral or alkaline region.
For example, the optimum pH of uricase produced by culturing an Alternaria tenuis strain is 7.
0 [Arch.
Microbiol.
, vol.
17.
255 (1952)].
Similarly, it is known that uricase having an optimum pH in the range of 8.
5-9.
5 may be produced from many microorganisms and the like.
For example, uricase is recoverable from rat liver [Biochemistry, vol.
13, 888 (1974)], Aspergillus flavus [C.
R.
Acad.
Sci
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Molecular Biology 
