 Article with a lyophilized immunoreactive self-adhering coating |
| I claim: 1. An article useful for immunological determinations which article comprises a carrier ... |
|
| Method, device and kit for measurement of tissue plasminogen activator activity |
| Namely, the present invention is characterized by reacting, a solid phase having bound thereto anti ... |
|
| Blocked cholecalciferol and dihydrotachysterol 3 derivatives |
| We have found that cholecalciferol and dihydrotachysterol.sub.3 and their derivatives may be ... |
|
| 4-[(Carboxyl- and sulfamyl-substituted alkyl)-amino] benzoic acids and analogs |
| OF THE INVENTION The compounds of the invention are new and novel 4-[(carboxyl- and sulfamyl-... |
|
| Vaccine production |
| What is claimed is: 1. In a process for the controlled manufacture of vaccines wherein cells are ... |
|
| Method of fermenting brewer's wort |
| We claim: 1. The method of preparing an alocholic beverage, comprising the steps of preparing an ... |
|
| Treatment of hemorrhagic shock |
| I claim: 1. A method of treating shock in humans to reverse the same and elevate blood pressure ... |
|
| Compositions comprising alkylpolysaccharide detergent surfactant |
| What is claimed is: 1. A homogeneous granular detergent composition comprising: (1) from about 2% ... |
|
| Process for the preparation of a mixture of sucrose oxidation products and the use thereof |
| We claim: 1. A process for the preparation of a mixture of sucrose oxidation products which ... |
|
|
Amylase determination
| Details |
Inventors: Driscoll, Richard Cornelius; Gargiulo, Robert James; Giegel, Joseph L.;
Assignee: American Hospital Supply Corporation (Evanston, IL)
Primary Examiner: Jones; Raymond N.
Assistant Examiner: Fan; C. A.
Attorney, Agent or Firm: Azulay; Y. Judd
A method for the determination of amylase, based on the cleavage of colorimetric, ultraviolet absorbing or fluorometric substances, commonly referred to as chromogenic substances as p-nitrophenol derivatives of oligosaccharides of chain length 4-10 glucose units. Oligosaccharides of this chain length are resistant to cleavage by .alpha. and/or .beta.-glucosidase and contain endo-.alpha.-1,4 linkages which are required for amylase activity. Cleavage of the .alpha.-1,4 bonds by amylase produces smaller fragments which are acted upon by .alpha.-glucosidase and/or .beta.-glucosidase to liberate a chromophore. The rate of appearance of the chromophore is proportional to amylase activity and lends itself to either rate or endpoint determinations. The qualitative or quantitative measurement of amylase concentrations in a sample is useful in medical diagnosis. |
|
DETAILED DESCRIPTION What is claimed is: 1.
A process for determining the .
alpha.
-amylase content of a sample comprising the steps of adding a chromogenic oligosaccharide substrate having 4-10 glucose units and a chromophore selected from the group of p-nitrophenol and 4-methylumbilliferone to a solution containing a measured amount of said sample and .
alpha.
and .
beta.
glucosidase and determining amylase activity by the release of a chromophore selected from the group of p-nitrophenol and 4-methylumbelliferone.
2.
The process of claim 1 wherein potassium phosphate is added to the solution as buffer.
Description:
SUMMARY OF THE INVENTION This invention relates to methods of determining amylase activity in biological fluids and more particularly to a novel method for the determination of amylase activity based on the cleavage of chromophore derivatives of oligosaccharides of chain length 4 to 10 glucose units.
BACKGROUND OF THE INVENTION One of the most widely studied and accepted procedures in clinical chemistry is the determination of serum and urine .
alpha.
-amylase which is used for the diagnosis of pancreatic disease.
During the past twenty-five years various amylase methods have been developed for use in the clinical laboratory.
Some of the methods, i.
e.
saccharogenic method, involves complicated methodology which makes its routine use prohibitive.
Other methods, i.
e.
, turbidometric and viscosimetric methods for the determination of .
alpha.
-amylase activity are dependent on changes in the physical properties of the substrate, which may be influenced to a considerable degree by other factors present in the serum.
Today, one of the most widely used methods for .
alpha.
-amylase determination is the starch-iodine method.
With this method only a specific portion of the substrate is measured and the enzyme does not work under substrate saturation conditions.
Further, the presence of serum proteins could interfere with the starch-iodine reaction.
In addition to the above difficulties associated with the mentioned methods, a further difficulty is encountered because the aforementioned methods can be used to determine a rather limited range of
|
| Related patents |
|
|
Process for producing dextrose using mixed immobilized enzymes
What is claimed is: 1. A process for converting starch to dextrose comprising treating starch with alpha-amylase to obtain a partially hydrolyzed starch solution ...
|
|
|
Continuous thermal reactor system and method
What is claimed is: 1. A continuous thermal reactor comprising a reactor vessel defining an elongated upright reaction chamber having a feed inlet in the upper portion ...
|
|
|
Somatostatin analogs
What is claimed is: 1. The peptides having the structure: ##STR55## wherein: X is CH.sub.2, S, ##STR56## SO.sub.2 and [X].sub.n wherein n=0; Y is CH.sub.2, S, ##STR57## S...
|
|
|
4-Styryl-hexahydro-4-indolinols
I claim: 1. A compound of formula I, ##STR7## wherein R.sub.1 and R.sub.2 are, independently, hydrogen, fluorine, chlorine, trifluoromethyl, alkyl of 1 to 4 carbon atoms,...
|
|
|
Derivatives of 1,3-benzodioxole, the preparation and use thereof
OF THE INVENTION It has been surprisingly found that the compounds of the general formula I are very active for the treatment of heart arrhythmia. Said compounds can be ...
|
|
|
Method of preparing L-(+)-.beta.-hydroxyisobutyric acid by fermentation
OF THE INVENTION The microorganism preferably employed in carrying out the method of the present invention will be Pseudomonas aeruginosa, for example, ATCC #15523 or ...
|
|
|
Six base oligonucleotide linkers and methods for their use
I claim: 1. A method for inserting a selected restriction site into a double stranded genetic sequence at a selected tab site comprising: treating a double stranded DNA ...
|
|
|
Immunoassay with chromatographic medium and labelled reagent
We claim: 1. A method of performing an immunoassay for an analyte in a sample, which method comprises providing a chromatographic medium, forming a reaction mixture ...
|
|
|
Stabilized urease composition
What is claimed is: 1. Stabilized urease composition comprising urease of a specific activity of from 25-1500 U/mg and, as a stabilizing agent, a stabilizing mixture of ...
|
|
|
Stabilized enzymic solutions for determining urea
OF THE INVENTION The stabilized enzyme solutions herein can be used in the clinical field for the determination of urea. The stabilized enzyme solutions herein can be ...
|
|
|
Molecular Biology 
