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 Bacterial nitrite oxidizer

Details
Inventors: Hovanec, Timothy A.;
Assignee: Aquaria, Inc. (Moorpark, CA)
Primary Examiner: Myers; Carla J.
Assistant Examiner: Johannsen; Diana
Attorney, Agent or Firm: Pillsbury Madison & Sutro LLP

The present invention provides an isolated bacterial strain capable of oxidizing nitrite to nitrate and a method of use thereof for preventing or alleviating the accumulation of nitrite in an aqueous medium.

DETAILED DESCRIPTION It is an object of the present invention to provide an isolated bacterium or bacterial strain capable of oxidizing nitrite to nitrate.
In a particularly preferred embodiment, the 16S rDNA of the bacterium or bacterial strain has the nucleotide sequence of SEQ ID NO:1, or a variant thereof which is at least 95% similar to SEQ ID NO:1.
The invention is also intended to include nucleic acid sequences and bacteria with sequences which are at least 90% similar, preferably 95% similar, most preferably 99% similar to SEQ ID NO:1.
For the purposes of this application, "95% similar" means that single base substitutions may occur of up to 5% of the bases.
By "99% similar" is meant that single base substitutions may occur of up to 1% of the bases.
In one preferred embodiment, the bacterium or bacterial strain will be in the form of a biologically pure culture of a bacterial strain capable of oxidizing nitrite to nitrate.
It is another object of the present invention to provide a mixture which includes a culture of concentrated bacteria capable of oxidizing nitrite to nitrate.
It is further object of the present invention to provide a method of alleviating the accumulation of nitrite in a medium.
The method comprises the addition to the medium of a bacterium or bacterial strain capable of oxidizing nitrite to nitrate.
It is yet another object of the present invention to provide a probe which can be used to detect and determine the quantity of bacteria capable of oxidizing nitrite to nitrate.
Accordingly, one object of the present invention is to provide a biologically pure culture of a bacterial strain capable of oxidizing nitrite to nitrate, wherein the 16S rDNA of the bacterial strain has a nucleotide sequence of SEQ ID NO:1.
Another object of the present invention is to provide a mixture comprising a concentrated bacteria capable of oxidizing nitrite to nitrate, wherein the 16S rDNA of the bacteria has a nucleotide sequence of SEQ ID NO:1.
A further aspect of the present invention is a method of alleviating the accumulation of nitrite in a water-containing medium



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