 Method of converting brines to useful products |
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| Method of and apparatus for filtering |
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| Subsurface waste disposal system |
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| Barrier for floating pollutants |
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| Apparatus for processing flushing liquor from coke ovens |
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| Coal suspensions in organic liquids |
| OF THE INVENTION In the former aforementioned U.S. Patent, coal dust suspensions in water provide ... |
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| Propagation of microbial cells on single carbon products |
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| Fermentation process for improved oxygen utilization |
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| Method of conditioning flue gas |
| OF THE PREFERRED EMBODIMENTS The conditioners useful in the present invention are finely divided ... |
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| Enzyme granules |
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Bioconversion process for the production of vanillin
| Details |
Inventors: Labuda, Ivica M.; Goers, Steven K.; Keon, Kathleen A.;
Assignee: Kraft General Foods, Inc. (Northfield, IL)
Primary Examiner: Lilling; Herbert J.
Assistant Examiner:
Attorney, Agent or Firm: Marcoux; Thomas A., Grim; Linn I., Savoie; Thomas R.
The present invention is directed to a process for the production of vanillin through the bioconversion of a vanillin precursor with a ferulic acid degrading microorganism in the presence of a water soluble sulfhydryl compound and optionally, also in the presence of an assimilable carbon source. |
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DETAILED DESCRIPTION We claim: 1.
A process for the production of vanillin comprising contacting a bioconversion mixture comprised of an aqueous solution of a vanillin precursor present in a concentration in the range of between about 0.
025% and 1.
0% having the structural formula ##STR3## wherein R is selected from --CH.
dbd.
CH--COOH, --CH.
dbd.
CH.
sub.
2, --CH.
sub.
2 --CH.
dbd.
CH.
sub.
2, --CH.
dbd.
CH--CH.
sub.
2 OH, --CH.
dbd.
CH--CHO, --CH.
dbd.
CH--CH.
sub.
2 OH, --COOH, --CH.
dbd.
CH--CH.
sub.
3, ##STR4## or --CH.
sub.
2 --CH(OH)--CH.
sub.
2 OH, with a ferulic acid degrading microorganism in the presence of a sulfhydryl compound selected from the group consisting of (a) polyhydric sulfhydryl compounds having the generic formula: HS--CH.
sub.
2 --(CH(OH)).
sub.
n --CH.
sub.
2 --SH wherein n is an integer of from 1 to 4, (b) beta-mercaptoethanol, (c) mercaptoacetic acid and (d) sulfur containing amino acids and recovering vanillin.
2.
The process according to claim 1 wherein the bioconversion mixture also contains an assimilable carbon source.
3.
The process according to claim 2 wherein the assimilable carbon source is selected from the group consisting of glucose, fructose, maltose, sucrose and combinations thereof.
4.
The process according to claim 1 wherein the vanillin precursor is selected from the group consisting of ferulic acid, eugenol, coniferyl alcohol and 4-vinylguaiacol.
5.
The process according to claim 1 wherein the sulfhydryl compound is represented by the formula: HS--CH.
sub.
2 --(CH(OH)).
sub.
n CH.
sub.
2 --SH wherein n is an integer of from 1 to 4.
6.
The process according to claim 1 wherein the sulfhydryl compound is a sulfur containing amine acid or derivative thereof.
7.
The process according to claim 1 wherein the sulfhydryl compound is selected from the group consisting of dithiothreitol, dithioerythritol, glutathione, cysteine and combinations thereof.
8.
The process according to claim 1 wherein the ferulic acid degrading microorganism is selected from the group consisting of Pseudomonas sp.
, Aspergillus sp
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Molecular Biology 
