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Biologically pure cultures of Pseudomonas sorbosoxidans useful for producing 2-keto-L-gulonic acid
| Details |
Inventors: Imai, Ko; Sakane, Takeshi; Nogami, Ikuo;
Assignee: Takeda Chemical Industries, Ltd. (Osaka, JP)
Primary Examiner: Lilling; Herbert J.
Assistant Examiner:
Attorney, Agent or Firm: Wenderoth, Lind & Ponack
2-Keto-L-gulonic acid is produced in a high yield by contacting L-sorbose with a microorganism of Pseudomonas sorbosoxidans, or a processed material thereof. |
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DETAILED DESCRIPTION OF THE INVENTION The three strains which the present inventors have found have the following taxonomic characteristics.
(a) Morphological characteristics: (1) Rods, 0.
3 to 0.
5.
times.
0.
7 to 1.
4 .
mu.
m.
(2) No cellular polymorphism observed.
(3) Having motility and 2 or more polar flagella.
(4) Non-sporulating.
(5) Gram-negative.
(6) Non-acid-fast.
(b) Cultural characteristics: (1) Nutrient agar plate: moderate growth; round, entire, smooth and milk-white colonies.
(2) Nutrient ager slant: moderate growth; filiform, smooth, milk-white.
(3) Nutrient liquid culture: moderate growth with precipitates.
(4) Nutrient gelatin stab culture: growth only at upper portion without liquefaction of gelatin.
(5) Litmus milk: acidified, not coagulated, not decomposed.
(c) Physiological characteristics: (1) Nitrate reduction: weakly positive.
(2) Denitrification: negative.
(3) Methyl red (MR) test: slightly positive.
(4) Voges-Proskauer (VP) test: slightly positive.
(5) Indole: not produced.
(6) Hydrogen sulfide: not produced.
(7) Starch hydrolysis: negative.
(8) Citrate utilization: negative.
(9) Ammonium salts: utilized as nitrogen source.
(10) Pigments: not produced.
(11) Urease: positive.
(12) Oxidase: positive.
(13) Catalase: positive.
(14) The strain can grow at 15.
degree.
to 36.
degree.
C.
The optima; growth temperature is about 30.
degree.
C.
The strain can grow at pH 5.
5 to 8.
7.
The optimum growth pH is 6.
0 to 7.
5.
(15) Aerobic.
(16) Hugh-Leifson's OF test: oxidative.
(17) Acid is slightly produced but gas is not produced from L-arabinose, D-xylose, D-glucose, D-mannose, D-fructose, D-galactose, maltose, sucrose, lactose, and trehalose.
Neither acid nor gas is produced from D-sorbitol, D-mannitol, inositol, glycerol, and starch.
(d) Other characteristics: (1) The content of guanine and cytosine of DNA: about 67 mole %.
(2) Containing ubiquinone with 10 isoprene units.
(3) Dihydroxyacetone: not produced from glycerol.
(4) Thiamine, riboflavine and pantothenic acid are essentially required for the growth
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Molecular Biology 
