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Citric acid producing mutant yeast strains
| Details |
Inventors: Takayama, Kenichiro; Adachi, Tetsuo; Kohata, Mamoru; Hattori, Kiyoji; Tomiyama, Tomoko;
Assignee: Kyowa Hakko Kogyo Co., Ltd. (Tokyo, JP)
Primary Examiner: Penland; R. B.
Assistant Examiner:
Attorney, Agent or Firm: Fitzpatrick, Cella, Harper & Scinto
Citric acid is selectively produced by fermentation of a mutant yeast strain which requires the presence of iron in the culture medium. Citric acid is accumulated in the culture medium and isolated therefrom. |
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DETAILED DESCRIPTION What is claimed is: 1.
A biologically pure culture of mutant yeast strain, Candida zeylanoides ATCC 20391, exhibiting growth substantially inferior to that of its parent strain in a minimum medium, but exhibiting growth comparable to that of its parent strain in a minimum medium including at least 0.
1 mg/L of iron ion or iron-containing ion, said mutant being capable of producing citric acid.
2.
A biologically pure culture of mutant yeast strain, Candida zeylanoides ATCC 20392, exhibiting growth substantially inferior to that of its parent strain in a minimum medium, but exhibiting growth comparable to that of its parent strain in a minimum medium including at least 0.
1 mg/L of iron ion or iron-containing ion, said mutant being capable of producing citric acid.
3.
A biologically pure culture of mutant yeast strain, Candida zeylanoides ATCC 20393, exhibiting growth substantially inferior to that of its parent strain in a minimum medium, but exhibiting growth comparable to that of its parent strain in a minimum medium including at least 0.
1 mg/L of iron ion or iron-containing ion, said mutant being capable of producing citric acid.
4.
A biologically pure culture of mutant yeast strain, Candida zeylanoides ATCC 20367, exhibiting growth substantially inferior to that of its parent strain in a minimum medium, but exhibiting growth comparable to that of its parent strain in a minimum medium including at least 0.
1 mg/L of iron ion or iron-containing ion, and about 0.
5 g/L of glycerin, said mutant being capable of producing citric acid.
Description:
BACKGROUND OF THE INVENTION The present invention relates to the selective production of citric acid and more specifically to the production of citric acid by fermentation of a mutant yeast strain which requires the presence of iron within the culture medium.
Citric acid has wide uses and is useful for example, for the preparation of beverages and pharmaceutical syrups.
Heretofore, there have been proposed in the art various processes for the production of citric acid using yeasts
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Molecular Biology 
