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Home Molecular Biology Cyanobacterium-produced-bioemulsifier-composition-and-solution-thereof

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 Cyanobacterium-produced bioemulsifier composition and solution thereof

Details
Inventors: Shilo, Moshe; Fattom, Ali;
Assignee: Solmat Systems, Ltd. (Yavne, IL); Yissum Research Development Company of the Hebrew University of Jerusalem (Jerusalem, IL)
Primary Examiner: Lovering; Richard D.
Assistant Examiner:
Attorney, Agent or Firm: Sandler & Greenblum

J-1 which is a strain of cyanobacteria is used to form and excrete a material useful as an emulsifying agent for forming emulsions of hydrocarbons and oils in liquids such as water. Method of separating and culturing the cyanobacteria under conditions necessary to achieve a maximum formation and excretion of the emulsifying agent into solution. Method of purifying and separating excreted as well as intracellular material from cyanobacteria. Method of removing stains with a material excreted by cyanobacteria, and particularly strain J-1. Method of effecting the secondary recovery of petroleum through the use of a material excreted by cyanobacteria, and particularly strain J-1. Extracellular polymeric material which is greater than 200,000 Daltons in molecular weight, and contains sugar, fatty acid, and protein moieties, and amide, carboxylic and amino groups.

DETAILED DESCRIPTION It is an object of the invention to provide a process for the production of a novel emulsifying agent, useful in the emulsification of hydrocarbons and oils.
The objective of the invention is achieved through the use of cellular products of a benthic filamentous cyanobacterium of Phormidium genus in the family Oscillatoriaceae.
This strain has been found to exist in a portion of the Jordan river, where it was noticed that the water seemed to be clarified of its particulates, as discussed in patent application Ser.
No.
634,535, filed July 26, 1984, and now U.
S.
Pat.
No.
4,649,110, of which this application is a continuation-in-part.
One particular bacterial strain of Phormidium useful in the invention has been denominated as J-1.
J-1, is, in effect, a definite strain of an undefined species of the Phormidium genus.
A specimen of this organism has been deposited with the American Type Culture Collection in Rockville, Md.
, and has been assigned ATCC No.
39161.
This strain has a generation time of 24-48 hours, and under the carefully controlled conditions of the invention, the organism produces and excretes a polymeric bioemulsifying material which is useful in achieving the objectives of the invention.
The polymeric bioemulsifier can also be secured from the bacterial cells themselves, by rupturing the cells and then isolating the bioemulsifying agent.
The organisms of the invention are found naturally in certain fresh water bodies.
These organisms can be collected and removed from the water for culturing, according to the inventive technique, by adding nonpolar hydrocarbons into the water containing the bacteria, and agitating the water to disperse the hydrocarbon into globules, as described in U.
S.
application Ser.
No.
634,535, now U.
S.
Pat.
No.
4,649,110 discussed above.
Because benthic bacteria (including members of the Phormidium genus) are hydrophobic, the bacteria become attached to the dispersed globules of hydrocarbon.
The hydrocarbon is collected as it floats to the water surface, and the bacteria can then be separated from the hydrocarbon and grown under laboratory conditions



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