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Cytochrome P450 monooxygenase and NADPH cytochrome P450 oxidoreductase genes and proteins related to the omega hydroxylase complex of Candida tropicalis and methods relating thereto
| Details |
Inventors: Wilson, C. Ron; Craft, David L.; Eirich, L. Dudley; Eshoo, Mark; Madduri, Krishna M.; Cornett, Cathy A.; Brenner, Alfred A.; Tang, Maria; Loper, John C.; Gleeson, Martin;
Assignee:
Primary Examiner: Achutamurthy; Ponnathapu
Assistant Examiner: Kerr; Kathleen
Attorney, Agent or Firm: Drach; John E., Steen; Jeffrey S.
Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the .omega.-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided. |
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DETAILED DESCRIPTION An isolated nucleic acid is provided which encodes a CPRA protein having the amino acid sequence set forth in SEQ ID NO: 83.
An isolated nucleic acid is also provided which includes a coding region defined by nucleotides 1006-3042 as set forth in SEQ ID NO: 81.
An isolated protein is provided which includes an amino acid sequence as set forth in SEQ ID NO: 83.
A vector is provided which includes a nucleotide sequence encoding CPRA protein including an amino acid sequence as set forth in SEQ ID NO: 83.
A host cell is provided which is transfected or transformed with the nucleic acid encoding CPRA protein having an amino acid sequence as set forth in SEQ ID NO: 83.
A method of producing a CPRA protein including an amino acid sequence as set forth in SEQ ID NO: 83 is also provided which includes a) transforming a suitable host cell with a DNA sequence that encodes the protein having the amino acid sequence as set forth in SEQ ID NO: 83; and b) culturing the cell under conditions favoring the expression of the protein.
An isolated nucleic acid is provided which encodes a CPRB protein having the amino acid sequence set forth in SEQ ID NO: 84.
An isolated nucleic acid is provided which includes a coding region defined by nucleotides 1033-3069 as set forth in SEQ ID NO: 82.
An isolated protein is provided which includes an amino acid sequence as set forth in SEQ ID NO: 84.
A vector is provided which includes a nucleotide sequence encoding CPRB protein including an amino acid sequence as set forth in SEQ ID NO: 84.
A host cell is provided which is transfected or transformed with the nucleic acid encoding CPRB protein having an amino acid sequence as set forth in SEQ ID NO: 84.
A method of producing a CPRB protein including an amino acid sequence as set forth in SEQ ID NO: 84 is provided which includes a) transforming a suitable host cell with a DNA sequence that encodes the protein having the amino acid sequence as set forth in SEQ ID NO: 84; and b) culturing the cell under conditions favoring the expression of the protein
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Molecular Biology 
