Home | Links | Contact Us | More About Intellectual Property | Bookmark
Search patents:
Home Molecular Biology Endo-B-1-4-glucanases-from-saccharothrix

 Thermosetting vinyl chloride polymer powder coatings
What I claim is: 1. A thermosetting vinyl chloride polymer powder coating composition comprising ...


 Worn tool detector utilizing normalized vibration signals
Pursuant to the present invention the signal waveform from a vibration sensor is connected to a ...


 Acoustic detection of tool break events in machine tool operations
An object of the invention is to provide a cutting tool break detection system that improves on ...


 TV service triggered oscilloscope
Briefly, there is provided in accordance with the present invention an oscilloscope having sweep ...


 Pulse generator having selectable pulse width and pulse repetition interval
The present invention overcomes the disadvantage and limitations of the prior art by providing an ...


 Process for making imidazolinium salts
OF THE INVENTION In accordance with this invention, quaternary imidazolinium salts essentially ...


 Genetically transformed pepper plants and methods for their production
The present invention comprises methods for the genetic transformation of pepper plant material and ...


 Sterilizing apparatus and integrated sterilizer control
We claim: 1. Sterilizing apparatus having a microcomputer control arrangement providing for ...


 Circuit for ventilating and filtering the medium contained in a confinement enclosure
We claim: 1. A process for establishing and maintaining a vacuum within a ventilated and filtered ...


 Apparatus for performing solution growth of group II-VI compound semiconductor crystal
It is, therefore, the object of the present invention to obviate the above-stated drawbacks of the ...


 Endo-B-1,4-glucanases from saccharothrix

Details
Inventors: Bj.o slashed.rnvad, Mads Eskelund; Hatakeyama, Mariko; Schulein, Martin; Nielsen, Jack Bech;
Assignee: NovoNordisk A/S (Bagsvaerd, DK)
Primary Examiner: Prouty; Rebecca E.
Assistant Examiner: Rao; Manjunath N.
Attorney, Agent or Firm: Laubiris, Esq.; Elisa J.

An enzyme preparation comprising an enzyme having endo-.beta.-1,4-glucanase activity obtainable from or endogeneous to a strain belonging to the genus Saccharothrix such as Saccharothrix australiensis, IFO 14444; an isolated polynucleotide (DNA) molecule encoding an enzyme or enzyme core (the catalytically active domain of the enzyme) exhibiting endo-.beta.-1,4-glucanase activity selected from (a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 676 to nucleotide 1470, (b) polynucleotide molecules that encode a polypeptide that is at least 80% identical to the amino acid sequence of SEQ ID NO:2 from amino acid residue 226 to amino acid residue 490, and (c) degenerate nucleotide sequences of (a) or (b), the expressed endoglucanase and the enzyme preparation being useful in a detergent or fabric softener composition or in the textile industry for improving the properties of cellulosic fibres or fabric or for providing a stone-washed look of denim.

DETAILED DESCRIPTION OF THE INVENTION The enzyme or enzyme preparation of the invention is obtainable from or endogeneous to a strain belonging to the genus Saccharothrix, preferably from the species selected from the group consisting of Saccharothrix australiensis, Saccharothrix texasensis, Saccharothrix waywayandensis, Saccharothrix cryophilis, Saccharothrix flava, Saccharothrix coeruleofusca, Saccharothrix longispora, Saccharothrix mutabilis ssp.
capreolus, Saccharothrix aerocolonigenes, Saccharothrix mutabilis ssp.
mutabilis, Saccharothrix syringae, Saccharothrix sp.
Examples of useful strains are Saccharothrix australiensis, IFO 14444, Saccharothrix texasensis, NRRL B-16134, Saccharothrix waywayandensis, NRRL B-16159, Saccharothrix cryophilis, NRRL B-16238, Saccharothrix sp.
, IFO 13785, Saccharothrix flava, ATCC 29533, Saccharothrix coeruleofusca (Actinomadura coeruleofusca), ATCC 35108 or DSM 43679, Saccharothrix longispora (Actinomadura longispora), ATCC 31109 or DSM 43749, Saccharothrix mutabilis ssp.
mutabilis, ATCC 31520, Saccharothrix aerocolonigenes, ATCC 23870, Saccharothrix mutabilis ssp.
capreolus, ATCC 23892, Saccharothrix mutabilis, DSM 40225 or DSM 43853, Saccharothrix syringae, DSM 43886.
The enzyme and the enzyme preparation of the invention is active over a broad pH range, preferably active at a pH between about 4 and about 11, preferably between about 5.
5 and about 10.
In a preferred embodiment, the enzyme or the enzyme preparation of the invention belongs to family 6 of glycosyl hydrolases (Henrissat et al.
, op.
cit.
).
In the present context the term "expression vector" denotes a DNA molecule, linear or circular, that comprises a segment encoding a polypeptide of interest operably linked to additional segments that provide for its transcription.
Such additional segments may include promoter and terminator sequences, and may optionally include one or more origins of replication, one or more selectable markers, an enhancer, a polyadenylation signal, and the like.
Expression vectors are generally derived from plasmid or viral DNA, or may contain elements of both



Related patents
  Stabilizer blend
The embodiments of the invention in which an exclusive property or privilege is claimed are defined as follows: 1. An ice cream composition which is stabilized and ...
  Production of a recombinant human colony stimulating factor
What is claimed is: 1. A process for producing a protein capable of stimulating proliferation of monocytic cells in both human and mouse bone marrow assays comprising ...
  Methyl isocyanate process
OF THE INVENTION This invention relates to a process for preparation of methyl isocyanate (MIC) by thermal dehydrochlorination of a solution of methyl carbamyl chloride ...
  Sulphonic acid salts of S-adenosilmethionine
I claim: 1. Stable salts of S-adenosil-L-methionine (SAM) with sulphonic acids selected from the group consisting of methanesulphonic, ethanesulphonic, 1-n-...
  2-Acrylamido-2-methylpropane sulfonic acid vinyl aminimide/copolymer
OF THE INVENTION The novel copolymers of the present invention are prepared by conventional vinyl polymerization techniques such as free radical polymerization ...
  Zearaline glycoside compounds
It is claimed: 1. A compound of the formula ##SPC11## wherein --A-- is either --CH.sub.2 --CH.sub.2 -- or --CH=CH--; >Z is either >CH.sub.2, >C=O or >CH--OH; ...
  Fortimicin AK
OF PREFERRED EMBODIMENTS The compound of this invention, fortimicin AK is represented by the Formula: ##STR1## Fortimicin AK is useful as an intermediate in the ...
  3-Cyano or acyl-4-aryl-5-arylazo-6-hydroxypyridone-2 dyes
1. A compound of the formula wherein R.sub.1 is phenyl, substituted phenyl, naphthyl or substituted naphthyl, wherein each substituent of substituted phenyl and ...
  Semi-rigid polyurethane foam used in packaging
What is claimed is: 1. A semi-rigid polyurethane foam prepared from a reaction mixture having an over-all index from about 30 to about 60, which mixture comprises: a. a ...
  Tripeptides having an antidepressive and prolactin-releasing action
What we claim is: 1. A tripeptide of the formula ##SPC2## wherein X is alkyl having 3 to 8 carbon atoms, cycloalkyl having 5 to 7 carbon atoms, or aralkyl having 1 or 2 ...

0.054

Archive: All patents - Links

Copyright (c)2006 Eipa-patents.org - All rights reserved