Enzymatic production of a starch conversion product having a high maltose content

DETAILED DESCRIPTION What we claim is: 1.
A method of enzymatic manufacturing of a starch conversion product having a high maltose content, using as a starting material a solution of a substrate comprising starch or a partial hydrolysate thereof comprising bringing the substrate solution into contact with a matrix consisting of a copolymer of acrylamide and acrylic acid to which both an .
alpha.
-1,6-glucosidase and an .
alpha.
-1,4-glucosidase having .
beta.
-amylase activity have been covalently coupled by a carbodiimide.
2.
The method of claim 1 wherein said .
alpha.
-1,6-glucosidase is pullulanase.
3.
The method of claim 1 wherein said .
alpha.
-1,6-glucosidase is isoamylase.
4.
A method according to claim 1 wherein said .
alpha.
-1,4-glucosidase is .
beta.
-amylase.
5.
A method according to claim 1 wherein said substrate is a gelatinized starch hydrolysed to a dextrose equivalent of between 0.
5 and 20 and wherein a starch hydrolysate containing at least 55% of maltose based on the dry content is obtained as an end product.
6.
A method according to claim 1 wherein said enzymatic treatment takes place continuously.
7.
A method according to claim 1 wherein the dry content of substrate amounts to between 10 and 40%.
8.
A method according to claim 1 wherein the pH-value of the substrate amounts to between 4.
5 and 8.
9.
A method according to claim 1 wherein said enzymatic treatment takes place at a temperature of between 30.
degree.
and 60.
degree.
C.
10.
A method according to claim 1 wherein the carbodiimide is 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide-metho-p-toluenesulphonate.




Description:
The present invention relates to a method of enzymatic production of a starch conversion product having a high maltose content using a solution of a substrate comprising starch or a partial hydrolysate thereof as a starting material.
It is already known to produce maltose using a starch solution or a solution of a hydrolysate thereof by subjecting it to the influence of an .
alpha.
-1,4-glucosidase having