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 Enzyme entrappment with cellulose acetate formulations

Details
Inventors: Emery, Alden H.; Lim, Henry C.; Kolarik, Michael J.;
Assignee: Purdue Research Foundation (West Lafayette, IN)
Primary Examiner: Naff; David M.
Assistant Examiner:
Attorney, Agent or Firm: Nesbitt; John R.

Enzymes are entrapped by preparing a polymer formulation containing cellulose triacetate and cellulose diacetate or glucose pentaacetate, adding an enzyme to the formulation, extruding the enzyme-containing formulation to form a solid matrix and soaking the solid matrix in an aqueous solution of acetone to swell and increase the permeability of the solid matrix.

DETAILED DESCRIPTION This invention provides an apparatus and method for entrapping enzymes and includes a reactor, formulations, and methods relating to chemical reactors containing immobilized enzymes, with particular formulations of cellulose acetate (including addition of second solid phases thereto) and subsequent swelling treatments increasing the apparent activity of the entrapped enzymatic material without decreasing the stability of the same.
It is therefore an object of this invention to provide an improved apparatus and method for entrapping enzymes.
It is another object of this invention to provide a chemical reactor containing immobilized enzymes.
It is another object of this invention to provide an improved reactor formulation including addition of second solid phases.
It is still another object of this invention to provide an improved method for forming a chemical reactor containing immobilized enzymes.
It is yet another object of this invention to provide a chemical reactor that includes a particular formulation subjected to subsequent swelling treatments to increase the apparent activity of entrapped enzymatic material without decreasing the stability of the same.
It is still another object of this invention to provide cellulose triacetate in solid form that has added thereto a second solid material and dissolved in methylene dichloride and extruded through an orifice into a toluene bath to form a fiber or film that is subjected to swelling to thereby increase the permeability of the fiber or film for superior performance.
With these and other objects in view which will become apparent to one skilled in the art as the description proceeds, this invention resides in the novel apparatus, reactor, formulation, and method, substantially as hereinafter described, and more particularly defined by the appended claims, it being understood that such changes in the precise embodiment of the herein disclosed invention is meant to be included as come within the scope of the claims.



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